Query Gene,In-Silico PCR, primer ID, chr:start, start, len, tm, gc%, any, 3', seq, product size 
sbf2, no result, ENSCAFT00000012037_ex1_e2F,33461566,109,20,59.8,45,2,0, aaccatccccgtcttttctt,698
sbf2, no result, ENSCAFT00000012037_ex1_e2R,33460869,806,18,60.58,66.67,3,1, ctcgtgtcgctccctcac,698
sbf2, no result, ENSCAFT00000012037_ex2F,33374512,2,23,59,39.13,4,2, tgcctctgtttatttttcctagc,487
sbf2, no result, ENSCAFT00000012037_ex2R,33374026,488,26,59.36,38.46,7,2, tcaaaggttactatacttggaagcag,487
sbf2, no result, ENSCAFT00000012037_ex3F,33264664,151,25,59.55,32,6,3, ggagggatatttaacatttcacaaa,383
sbf2, no result, ENSCAFT00000012037_ex3R,33264282,533,22,58.96,40.91,3,0, tttcttcctgtcttctccaaca,383
sbf2, no result, ENSCAFT00000012037_ex4F,33243389,69,23,58.13,30.43,3,2, ttgctttttagattcctgtcaaa,463
sbf2, no result, ENSCAFT00000012037_ex4R,33242927,531,23,59.24,30.43,4,2, tcatccaacatgaacacattttt,463
sbf2, no result, ENSCAFT00000012037_ex5F,33241496,160,20,60.81,60,6,2, ccctaggagcagatgtcacg,340
sbf2, no result, ENSCAFT00000012037_ex5R,33241157,499,20,60.07,55,4,2, gaaggcagaccatagggaca,340
sbf2, no result, ENSCAFT00000012037_ex6F,33239843,37,21,59.23,42.86,5,1, tttgggcacagtgtaagttga,496
sbf2, no result, ENSCAFT00000012037_ex6R,33239348,532,20,60.56,50,5,1, ggccgccctagagttaaaaa,496
sbf2, no result, ENSCAFT00000012037_ex7F,33213348,114,20,60.39,60,1,1, tccctcctcctgtgtctctg,362
sbf2, no result, ENSCAFT00000012037_ex7R,33212987,475,20,59.71,50,5,2, gcccttgaggttcaaaactg,362
sbf2, no result, ENSCAFT00000012037_ex8F,33212027,1,20,60.23,55,5,2, agggataagggagctgctgt,459
sbf2, no result, ENSCAFT00000012037_ex8R,33211569,459,20,60.24,45,4,2, tgggaatgcaacagaactga,459
sbf2, no result, ENSCAFT00000012037_ex9F,33209579,122,20,60.39,55,7,1, ggacagccaagagtcttcca,324
sbf2, no result, ENSCAFT00000012037_ex9R,33209256,445,25,59.31,32,8,1, tttaaagtaagcatactgccaatga,324
sbf2, no result, ENSCAFT00000012037_ex10F,33206508,89,21,59.66,52.38,6,2, gagcacctcagctaactgcat,401
sbf2, no result, ENSCAFT00000012037_ex10R,33206108,489,20,60.25,55,3,0, cctctgggagcaggtaacaa,401
sbf2, no result, ENSCAFT00000012037_ex11F,33205447,7,20,60.22,45,3,3, ctttgtttggctcccttgaa,452
sbf2, no result, ENSCAFT00000012037_ex11R,33204996,458,21,60.09,42.86,6,0, ggtcaaaatgcatggctagaa,452
sbf2, no result, ENSCAFT00000012037_ex12F,33204610,43,24,60.29,37.5,4,2, tctcctgctttatcaacaaactca,414
sbf2, no result, ENSCAFT00000012037_ex12R,33204197,456,26,57.77,30.77,6,1, ggatcttgattaaaataccatttctc,414
sbf2, no result, ENSCAFT00000012037_ex13F,33201482,88,20,60.09,45,3,0, gaggtgaaacccagcaaaaa,393
sbf2, no result, ENSCAFT00000012037_ex13R,33201090,480,20,59.69,55,4,1, ctccatgcttagtggggaag,393
sbf2, no result, ENSCAFT00000012037_ex14F,33189975,147,19,59.81,52.63,4,1, ttcagtttgtggccctctg,439
sbf2, no result, ENSCAFT00000012037_ex14R,33189537,585,20,59.65,45,4,2, caatgctccaattctggtca,439
sbf2, no result, ENSCAFT00000012037_ex15F,33184090,97,20,60.1,50,4,2, accttcgatgctatgcaacc,492
sbf2, no result, ENSCAFT00000012037_ex15R,33183599,588,20,60.01,55,2,0, cttctccctttccctccatc,492
sbf2, no result, ENSCAFT00000012037_ex16F,33182455,39,20,57.05,35,4,2, tttttcatgctggcttacaa,454
sbf2, no result, ENSCAFT00000012037_ex16R,33182002,492,22,60.76,45.45,6,3, cggaaggcttaagaacttgaca,454
sbf2, no result, ENSCAFT00000012037_ex17F,33127730,68,22,58.93,36.36,5,0, tgcttcaaggacagttttgttt,427
sbf2, no result, ENSCAFT00000012037_ex17R,33127304,494,20,60.33,50,4,1, ggacccatgcagttcaaatc,427
sbf2, no result, ENSCAFT00000012037_ex18F,33091179,55,20,59.98,50,6,0, gcttcatagcaagcccaaag,497
sbf2, no result, ENSCAFT00000012037_ex18R,33090683,551,20,60.26,60,5,2, gctcaccacaggactcccta,497
sbf2, no result, ENSCAFT00000012037_ex19F,33080380,146,26,60.03,34.62,7,3, ttggtgacaggtatttaatagatgga,442
sbf2, no result, ENSCAFT00000012037_ex19R,33079939,587,21,59.52,42.86,4,3, tgcacagcagtatttgcctaa,442
sbf2, no result, ENSCAFT00000012037_ex20F,33078012,95,20,59.56,55,5,3, ggacaatgcccgatagtagc,444
sbf2, no result, ENSCAFT00000012037_ex20R,33077569,538,21,60.24,47.62,5,2, gcatggctaagctacatggaa,444
sbf2, no result, ENSCAFT00000012037_ex21F,33077437,91,20,60.2,45,2,2, tgggaaacaaacaaggggta,304
sbf2, no result, ENSCAFT00000012037_ex21R,33077134,394,20,59.99,55,3,3, ctgggggcacaacagtctat,304
sbf2, no result, ENSCAFT00000012037_ex22F,33074788,93,20,60.33,50,4,2, catttctgcctcttcccaga,464
sbf2, no result, ENSCAFT00000012037_ex22R,33074325,556,24,60.38,41.67,5,1, cctggtgataaaaggcttactttg,464
sbf2, no result, ENSCAFT00000012037_ex23F,33071245,118,20,60.85,50,5,0, attcctgggcattgtcctct,426
sbf2, no result, ENSCAFT00000012037_ex23R,33070820,543,20,60.08,50,4,1, ccaaagggttcaggaagtga,426
sbf2, no result, ENSCAFT00000012037_ex24F,33069989,36,20,60.05,45,6,0, ttccttgccagaaaaccatc,497
sbf2, no result, ENSCAFT00000012037_ex24R,33069493,532,20,59.99,50,3,3, gcttgacagaatggcagaca,497
sbf2, no result, ENSCAFT00000012037_ex25F,33067768,147,21,59.61,47.62,4,2, tggagaagaaacgagttgagc,384
sbf2, no result, ENSCAFT00000012037_ex25R,33067385,530,20,59.88,50,4,0, aagctggtaagtgcctccaa,384
sbf2, no result, ENSCAFT00000012037_ex26F,33065036,127,20,60.1,50,3,2, aaagtgcctggagccctaat,485
sbf2, no result, ENSCAFT00000012037_ex26R,33064552,611,20,60.13,50,4,2, aggagatgcttgctcatgct,485
sbf2, no result, ENSCAFT00000012037_ex27F,33053693,161,20,60.3,50,2,0, cctccccatttctttcctgt,401
sbf2, no result, ENSCAFT00000012037_ex27R,33053293,561,20,60.55,55,4,3, agcctgcatgtgagagaagg,401
sbf2, no result, ENSCAFT00000012037_ex28F,33051209,109,20,59.71,45,3,1, tttgagtggggcaagtttct,478
sbf2, no result, ENSCAFT00000012037_ex28R,33050732,586,20,59.93,45,4,0, ctgaatccctgcatttggtt,478
sbf2, no result, ENSCAFT00000012037_ex29F,33040902,15,20,59.93,55,4,1, aggtccctccaaccctaaga,496
sbf2, no result, ENSCAFT00000012037_ex29R,33040407,510,22,58.19,36.36,6,3, tttctaggaaagctggtttgaa,496
sbf2, no result, ENSCAFT00000012037_ex30F,33037159,109,20,59.99,55,4,0, tcctgacctctgcaacctct,419
sbf2, no result, ENSCAFT00000012037_ex30R,33036741,527,20,60.31,55,3,2, tgtcaggtagtgagggcaca,419
sbf2, no result, ENSCAFT00000012037_ex31F,33034947,48,22,60.38,36.36,7,0, tgattttgacaaagaaccacca,461
sbf2, no result, ENSCAFT00000012037_ex31R,33034487,508,20,59.86,50,5,3, gctcagggcacaaaactttc,461
sbf2, no result, ENSCAFT00000012037_ex32F,33034072,135,20,59.73,55,5,2, tgtcctggagcagctagtga,420
sbf2, no result, ENSCAFT00000012037_ex32R,33033653,554,20,59.98,55,8,3, gcaagccttatggctgagtc,420
sbf2, no result, ENSCAFT00000012037_ex33F,33018608,139,20,59.93,50,8,2, gaccaaatggtctcctgcat,330
sbf2, no result, ENSCAFT00000012037_ex33R,33018279,468,20,60.99,45,5,3, cctaaattggctttgccaca,330
sbf2, no result, ENSCAFT00000012037_ex34F,33016319,131,20,59.85,50,3,2, gccgttttgagagccataag,398
sbf2, no result, ENSCAFT00000012037_ex34R,33015922,528,20,59.72,50,6,3, gcccatggtgtaccaaactt,398
sbf2, no result, ENSCAFT00000012037_ex35F,33014967,153,20,60.6,50,4,2, tgccctagaccctgcaaata,471
sbf2, no result, ENSCAFT00000012037_ex35R,33014497,623,20,60.08,50,4,1, ttggtttcctcaacctcctg,471
sbf2, no result, ENSCAFT00000012037_ex36F,33013777,65,20,60.01,55,4,2, tgcatcacaccagctagagg,413
sbf2, no result, ENSCAFT00000012037_ex36R,33013365,477,20,60.48,50,3,1, gaactttttgccctggacct,413
sbf2, no result, ENSCAFT00000012037_ex37F,33011926,107,20,60.54,60,3,3, ctctctctctccccgtgtga,473
sbf2, no result, ENSCAFT00000012037_ex37R,33011454,579,21,59.43,42.86,4,3, ttcaaagagccatctgacaca,473
sbf2, no result, ENSCAFT00000012037_ex38F,33010840,127,20,60.04,50,5,0, ttggaccatctgagggaaag,440
sbf2, no result, ENSCAFT00000012037_ex38R,33010401,566,20,60.16,55,4,2, agtgtggggcttgaactcac,440
sbf2, no result, ENSCAFT00000012037_ex39F,33008925,145,20,59.95,55,5,3, caggcaggggttagtatcca,426
sbf2, no result, ENSCAFT00000012037_ex39R,33008500,570,20,59.99,55,6,1, tctgaacagctgggagaggt,426
sbf2, no result, ENSCAFT00000012037_ex40_e1F,33007780,130,20,60.07,50,3,0, ttctggggcagtatggaaag,683
sbf2, no result, ENSCAFT00000012037_ex40_e1R,33007098,812,20,59.98,55,8,2, gctattctgcggagaacctg,683