Figure 3 The expression of genes involved in hemogobin biosynthesis and degradation in the spleen of thalassemic mice,,,,,,,,,,,
,,,,,,,,,,,
,,,delta Ct,,,,,,,,
TfR1 mRNA expression in the spleen,Average mActb Cq,Averag mTfrc Cq,Cq mTfrc - Cq mActb,,,,,,,,
WT 6-7 weeks old,20.39,28.85,8.45,,,,,,,,
,19.93,28.32,8.39,,,,,,,,
,20.24,30.29,10.06,,,,,,,,
,19.10,29.09,9.99,,,,,,,,
,19.64,26.67,7.03,,,,,,,,
BKO 6-7 weeks old,20.61,25.22,4.61,,,,,,,,
,20.63,24.98,4.35,,,,,,,,
,21.25,25.30,4.05,,,,,,,,
,21.30,26.43,5.13,,,,,,,,
,20.92,25.05,4.13,,,,,,,,
WT 16-20 weeks old,22.48,33.36,10.88,,,,,,,,
,21.57,32.68,11.12,,,,,,,,
,25.06,33.50,8.44,,,,,,,,
,20.19,29.67,9.48,,,,,,,,
,20.69,31.79,11.09,,,,,,,,
BKO 16-20 weeks old,20.10,23.76,3.66,,,,,,,,
,20.80,24.57,3.77,,,,,,,,
,20.34,24.32,3.98,,,,,,,,
,20.82,25.29,4.46,,,,,,,,
,21.29,25.14,3.85,,,,,,,,
,,,,,,,,,,,
,,,delta Ct,,,,,,,,
Mfrn1 mRNA expression in the spleen,Average mActb Cq,Averag mSlc25a37 Cq,Cq mSlc25a37 - Cq mActb,,,,,,,,
WT 6-7 weeks old,20.39,28.92,8.53,,,,,,,,
,19.93,29.44,9.51,,,,,,,,
,20.24,29.90,9.66,,,,,,,,
,19.10,28.85,9.75,,,,,,,,
,19.64,26.52,6.88,,,,,,,,
BKO 6-7 weeks old,20.61,24.49,3.88,,,,,,,,
,20.63,23.90,3.27,,,,,,,,
,21.25,25.00,3.76,,,,,,,,
,21.30,25.55,4.25,,,,,,,,
,20.92,24.45,3.53,,,,,,,,
"WT 16-20 weeks ol,d",22.41,33.34,10.93,,,,,,,,
,21.64,33.91,12.27,,,,,,,,
,25.33,36.16,10.83,,,,,,,,
,20.28,31.02,10.74,,,,,,,,
,20.70,32.51,11.80,,,,,,,,
BKO 16-20 weeks old,20.10,24.34,4.24,,,,,,,,
,20.80,25.18,4.37,,,,,,,,
,20.34,25.23,4.89,,,,,,,,
,20.82,26.11,5.29,,,,,,,,
,21.29,25.73,4.43,,,,,,,,
,,,,,,,,,,,
,,,delta Ct,,,,,,,,
Alas2 mRNA expression in the spleen,Average mActb Cq,Averag mAlas2 Cq,Cq mAlas2 - Cq mActb,,,,,,,,
WT 6-7 weeks old,20.39,25.98,5.58,,,,,,,,
,19.93,25.37,5.44,,,,,,,,
,20.24,26.65,6.41,,,,,,,,
,19.10,25.29,6.19,,,,,,,,
,19.64,23.92,4.28,,,,,,,,
BKO 6-7 weeks old,20.61,22.97,2.36,,,,,,,,
,20.63,22.64,2.01,,,,,,,,
,21.25,22.71,1.47,,,,,,,,
,21.30,24.02,2.72,,,,,,,,
,20.92,22.17,1.25,,,,,,,,
WT 16-20 weeks old,22.41,29.37,6.96,,,,,,,,
,21.64,29.02,7.38,,,,,,,,
,20.28,26.41,6.13,,,,,,,,
,20.70,29.29,8.58,,,,,,,,
BKO 16-20 weeks old,20.10,23.24,3.14,,,,,,,,
,20.80,23.96,3.15,,,,,,,,
,20.34,22.60,2.26,,,,,,,,
,20.82,23.23,2.40,,,,,,,,
,21.29,23.18,1.89,,,,,,,,
,,,,,,,,,,,
,,,delta Ct,,,,,,,,
HO-1 mRNA expression in the spleen,Average mActb Cq,Averag mHmox1 Cq,Cq mHmox1 - Cq mActb,,,,,,,,
WT 6-7 weeks old,20.39,28.03,7.63,,,,,,,,
,19.93,27.19,7.26,,,,,,,,
,20.24,27.65,7.41,,,,,,,,
,19.10,26.17,7.07,,,,,,,,
,19.64,26.23,6.60,,,,,,,,
BKO 6-7 weeks old,20.61,25.25,4.64,,,,,,,,
,20.63,25.44,4.81,,,,,,,,
,21.25,26.09,4.84,,,,,,,,
,21.30,26.18,4.88,,,,,,,,
,20.92,24.86,3.94,,,,,,,,
WT 16-20 weeks old,22.41,31.98,9.57,,,,,,,,
,21.64,31.27,9.63,,,,,,,,
,20.28,29.27,9.00,,,,,,,,
,20.70,29.01,8.30,,,,,,,,
BKO 16-20 weeks old,20.10,24.62,4.51,,,,,,,,
,20.80,25.56,4.76,,,,,,,,
,20.34,25.19,4.85,,,,,,,,
,20.82,25.42,4.60,,,,,,,,
,21.29,25.61,4.32,,,,,,,,
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