Supplemental Data 1: Qubit concentrations and results from two qPCR runs of P. clarkii eDNA samples run for another species (Corbicula fluminea),,,,,,,,,,,,,,,,,,,,
Qubit® dsDNA HS Assay Kit,,,,,,Plate: Pclarkii_Corbicula_10Feb2020,,,,,,,,Plate: Pclarkii_Corbicula_12Feb2020,,,,,,
Date,Location,Concentration (ng/µl),,,,Well,Plate Label,Water sample,Amp/No Amp,Quantity,# copies,Standard curve: 1pg-100zg,,Well,Sample,Water sample,Amp/No Amp,Quantity,# copies,Standard curve: 1pg-100zg
3-Jan,Extraction blank 1,<0.0005,,,,A1,9/14 B-1,9/14 Field blank ,No Amp,0,0,1ag: 3/3 amps,,A1,1/4 EB-1,Extraction blank,No Amp,0,0,100zg: 3/3
3-Jan,Extraction blank 2,<0.0005,,,,A2,9/14 B-1,9/14 Field Blank -2,No Amp,0,0,100zg: 2/3 amps,,A2,1/4 EB-1,Extraction blank,No Amp,0,0,R2=0.996
3-Jan,Extraction blank 3,<0.0005,,,,A3,9/14 B-1,9/14 Field Blank -3,No Amp,0,0,R2: 0.999,,A3,1/4 EB-1,Extraction blank,No Amp,0,0,Slope: -3.426
4-Jan,Extraction blank 1,<0.0005,,,,A4,9/17 A-M,9/17 Above-middle,Amp,9.82E-06,43.74,Slope:-3.474,,A4,9/17 C3,9/17 Cage-3,Amp,9.49E-06,42.26980769,Y-inter: 12.675
4-Jan,Extraction blank 2,<0.0005,,,,A5,9/17 A-M,9/17 Above-middle,Amp,7.63E-06,33.99,Y-Inter: 12.622,,A5,9/17 C3,9/17 Cage-3,Amp,8.82E-06,39.28553255,Eff%: 95.814
4-Jan,Extraction blank 3,<0.0005,,,,A6,9/17 A-M,9/17 Above-middle,Amp,9.70E-06,43.21,Eff%: 94.019,,A6,9/17 C3,9/17 Cage-3,Amp,8.87E-06,39.50823965,Error: 0.044
7-Jan,Extraction blank 1,<0.0005,,,,A7,9/17 BM-2,9/17 Below-middle 2,Amp,1.49E-05,66.37,Error: 0.026,,A7,9/21 BM2,9/21 Below-middle 2,Amp,1.83E-06,8.151079882,
7-Jan,Extraction blank 2,<0.0005,,,,A8,9/17 BM-2,9/17 Below-middle 3,Amp,1.73E-05,77.06,From the ThermoFisher DNA number and dilution calculator:,,A8,9/21 BM2,9/21 Below-middle 2,Amp,2.03E-06,9.041908284,
7-Jan,Extraction blank 3,<0.0005,,,,A9,9/17 BM-2,9/17 Below-middle 4,Amp,1.55E-05,69.04, 4454142.012 copies/1 pg,,A9,9/21 BM2,9/21 Below-middle 2,Amp,2.16E-06,9.620946746,
7-Jan,Extraction blank 4,<0.0005,,,,B1,9/14 B-2,9/14 Field blank 2,No Amp,0,0,https://www.thermofisher.com/us/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/thermo-scientific-web-tools/dna-copy-number-calculator.html,,B1,1/4 EB-2,Extraction blank,No Amp,0,0,
14-Sep,Field blank 1,<0.0005,,,,B2,9/14 B-2,9/14 Field blank 2,No Amp,0,0,,,B2,1/4 EB-2,Extraction blank,No Amp,0,0,
14-Sep,Field blank 2,<0.0005,,,,B3,9/14 B-2,9/14 Field blank 2,No Amp,0,0,,,B3,1/4 EB-2,Extraction blank,No Amp,0,0,
17-Sep,Field blank 1,<0.0005,,,,B4,9/17 A-L,9/17 Above-left,Amp,7.38E-07,3.29,,,B4,9/17 C4,9/17 Cage-4,Amp,2.45E-06,10.91264793,
17-Sep,Field blank 2,<0.0005,,,,B5,9/17 A-L,9/17 Above-left,Amp,5.04E-07,2.24,,,B5,9/17 C4,9/17 Cage-4,Amp,1.58E-06,7.037544379,
21-Sep,Field blank 1,<0.0005,,,,B6,9/17 A-L,9/17 Above-left,Amp,1.95E-07,0.87,,,B6,9/17 C4,9/17 Cage-4,Amp,9.69E-07,4.31606361,
21-Sep,Field blank 2,<0.0005,,,,B7,9/17 B-R,9/17 Below-right,Amp,4.04E-06,17.99,,,B7,9/21 BL,9/21 Below-left,Amp,1.99E-06,8.863742604,
28-Sep,Field blank 1,<0.0005,,,,B8,9/17 B-R,9/17 Below-right,Amp,4.33E-06,19.29,,,B8,9/21 BL,9/21 Below-left,Amp,2.53E-06,11.26897929,
28-Sep,Field blank 2,<0.0005,,,,B9,9/17 B-R,9/17 Below-right,Amp,4.29E-06,19.11,,,B9,9/21 BL,9/21 Below-left,Amp,3.22E-06,14.34233728,
5-Oct,Field blank 1,<0.0005,,,,C1,9/17 B-1,9/17 Field blank 1,No Amp,0,0,,,C1,1/7 EB-2,Extraction blank,No Amp,0,0,
5-Oct,Field blank 2,<0.0005,,,,C2,9/17 B-1,9/17 Field blank 1,No Amp,0,0,,,C2,1/7 EB-2,Extraction blank,No Amp,0,0,
12-Oct,Field blank 1,<0.0005,,,,C3,9/17 B-1,9/17 Field blank 1,No Amp,0,0,,,C3,1/7 EB-2,Extraction blank,No Amp,0,0,
12-Oct,Field blank 2,<0.0005,,,,C4,9/14 B-L,9/14 Below-left,Amp,2.71E-07,1.21,,,C4,9/17 C5,9/17 Cage-5,Amp,1.22E-06,5.434053255,
15-Oct,Field blank 1,<0.0005,,,,C5,9/14 B-L,9/14 Below-left,Amp,4.55E-07,2.03,,,C5,9/17 C5,9/17 Cage-5,Amp,1.05E-06,4.676849113,
15-Oct,Field blank 2,<0.0005,,,,C6,9/14 B-L,9/14 Below-left,Amp,7.65E-07,3.41,,,C6,9/17 C5,9/17 Cage-5,Amp,1.07E-06,4.765931953,
19-Oct,Field blank 1,<0.0005,,,,C7,9/14 C1,9/14 Cage-1,Amp,9.85E-06,43.87,,,C7,9/21 C1,9/21  Cage-1,Amp,3.85E-06,17.14844675,
19-Oct,Field blank 2,<0.0005,,,,C8,9/14 C1,9/14 Cage-1,Amp,1.25E-05,55.68,,,C8,9/21 C1,9/21  Cage-1,Amp,3.16E-06,14.07508876,
14-Sep,Above-right,0.0807,,,,C9,9/14 C1,9/14 Cage-1,Amp,1.19E-05,53,,,C9,9/21 C1,9/21  Cage-1,Amp,4.31E-06,19.19735207,
14-Sep,Above-middle,0.649,,,,D1,9/17 B-2,9/17 Field blank 2,No Amp,0,0,,,D1,1/7 EB-3,Extraction blank,No Amp,0,0,
14-Sep,Above-left,1.9,,,,D2,9/17 B-2,9/17 Field blank 2,No Amp,0,0,,,D2,1/7 EB-3,Extraction blank,No Amp,0,0,
17-Sep,Above-right,0.587,,,,D3,9/17 B-2,9/17 Field blank 2,No Amp,0,0,,,D3,1/7 EB-3,Extraction blank,No Amp,0,0,
17-Sep,Above-middle,1.63,,,,D4,9/14 BM-1,9/14 Below-middle 1,Amp,3.92E-07,1.75,,,D4,9/21 AR,9/21 Above-right,Amp,2.59E-06,11.53622781,
17-Sep,Above-left,0.112,,,,D5,9/14 BM-1,9/14 Below-middle 1,Amp,1.71E-06,7.62,,,D5,9/21 AR,9/21 Above-right,Amp,3.07E-06,13.67421598,
21-Sep,Above-right,1.12,,,,D6,9/14 BM-1,9/14 Below-middle 1,Amp,2.69E-06,11.98,,,D6,9/21 AR,9/21 Above-right,Amp,2.89E-06,12.87247041,
21-Sep,Above-middle,1.6,,,,D7,9/14 C2,9/14 Cage-2,Amp,6.82E-06,30.38,,,D7,9/21 C2,9/21 Cage-2,Amp,8.07E-06,35.94492604,
21-Sep,Above-left,3.68,,,,D8,9/14 C2,9/14 Cage-2,Amp,6.33E-06,28.19,,,D8,9/21 C2,9/21 Cage-2,Amp,8.43E-06,37.54841716,
28-Sep,Above-right,1.02,,,,D9,9/14 C2,9/14 Cage-2,Amp,6.22E-06,27.7,,,D9,9/21 C2,9/21 Cage-2,Amp,1.06E-05,47.21390533,
28-Sep,Above-middle,0.693,,,,E1,9/14 A-R,9/14 Above-right,Amp,1.16E-07,0.52,,,E1,9/21 B1,9/21 Field blank 1,No Amp,0,0,
28-Sep,Above-left,3.39,,,,E2,9/14 A-R,9/14 Above-right,Amp,7.52E-07,3.35,,,E2,9/21 B1,9/21 Field blank 1,No Amp,0,0,
4-Oct,Above-right,4.37,,,,E3,9/14 A-R,9/14 Above-right,No Amp,0,0,,,E3,9/21 B1,9/21 Field blank 1,No Amp,0,0,
4-Oct,Above-middle,0.727,,,,E4,9/14 BM-2,9/14 Below-middle 2,Amp,3.70E-06,16.48,,,E4,9/21 AM,9/21 Above-middle,Amp,1.92E-06,8.551952663,
4-Oct,Above-left,1.83,,,,E5,9/14 BM-2,9/14 Below-middle 2,Amp,2.97E-06,13.23,,,E5,9/21 AM,9/21 Above-middle,Amp,2.72E-06,12.11526627,
12-Oct,Above-right,0.773,,,,E6,9/14 BM-2,9/14 Below-middle 2,Amp,3.32E-06,14.79,,,E6,9/21 AM,9/21 Above-middle,Amp,2.11E-06,9.398239645,
12-Oct,Above-middle,0.733,,,,E7,9/14 C3,9/14 Cage-3,Amp,1.31E-05,58.35,,,E7,9/21 C3,9/21 Cage-3,Amp,7.48E-06,33.31698225,
12-Oct,Above-left,0.553,,,,E8,9/14 C3,9/14 Cage-3,Amp,1.12E-05,49.89,,,E8,9/21 C3,9/21 Cage-3,Amp,8.44E-06,37.59295858,
15-Oct,Above-right,0.316,,,,E9,9/14 C3,9/14 Cage-3,No Amp,0,0,,,E9,9/21 C3,9/21 Cage-3,Amp,9.84E-06,43.8287574,
15-Oct,Above-middle,0.215,,,,F1,9/14 A-M,9/14 Above-middle,No Amp,0,0,,,F1,9/21 B2,9/21 Field blank 2,No Amp,0,0,
15-Oct,Above-left,0.121,,,,F2,9/14 A-M,9/14 Above-middle,Amp,3.39E-06,15.1,,,F2,9/21 B2,9/21 Field blank 2,No Amp,0,0,
19-Oct,Above-right,0.258,,,,F3,9/14 A-M,9/14 Above-middle,Amp,3.29E-06,14.65,,,F3,9/21 B2,9/21 Field blank 2,No Amp,0,0,
19-Oct,Above-middle,0.193,,,,F4,9/14 B-R,9/14 Below-right,Amp,7.06E-06,31.45,,,F4,9/21 AL,9/21 Above-left,Amp,5.59E-06,24.89865385,
19-Oct,Above-left,0.84,,,,F5,9/14 B-R,9/14 Below-right,Amp,9.48E-06,42.23,,,F5,9/21 AL,9/21 Above-left,Amp,8.85E-06,39.41915681,
14-Sep,Below-left,0.098,,,,F6,9/14 B-R,9/14 Below-right,No Amp,0,0,,,F6,9/21 AL,9/21 Above-left,Amp,1.88E-05,83.73786983,
14-Sep,Below-middle 1,0.306,,,,F7,9/14 C4,9/14 Cage-4,Amp,1.92E-05,85.52,,,F7,9/21 C4,9/21 Cage-4,Amp,5.51E-06,24.54232249,
14-Sep,Below-middle 2,0.339,,,,F8,9/14 C4,9/14 Cage-4,Amp,1.64E-05,73.05,,,F8,9/21 C4,9/21 Cage-4,Amp,6.55E-05,291.7463018,
14-Sep,Below-right,1.98,,,,F9,9/14 C4,9/14 Cage-4,Amp,1.44E-05,64.14,,,F9,9/21 C4,9/21 Cage-4,Amp,5.39E-06,24.00782544,
17-Sep,Below-left,1.22,,,,G1,9/14 A-L,9/14 Above-left,Amp,3.67E-07,1.63,,,G1,9/17 C1,9/17 Cage-1,Amp,1.74E-05,77.50207101,
17-Sep,Below-middle 1,1.77,,,,G2,9/14 A-L,9/14 Above-left,Amp,7.84E-06,34.92,,,G2,9/17 C1,9/17 Cage-1,Amp,1.80E-05,80.17455622,
17-Sep,Below-middle 2,1.79,,,,G3,9/14 A-L,9/14 Above-left,Amp,8.63E-06,38.44,,,G3,9/17 C1,9/17 Cage-1,Amp,2.36E-05,105.1177515,
17-Sep,Below-right,0.7,,,,G4,9/17 B-L,9/17 Below-left,Amp,5.90E-06,26.28,,,G4,9/21 BR,9/21 Below-right,Amp,1.74E-06,7.750207101,
21-Sep,Below-left,1.05,,,,G5,9/17 B-L,9/17 Below-left,Amp,5.44E-06,24.23,,,G5,9/21 BR,9/21 Below-right,Amp,1.80E-06,8.017455622,
21-Sep,Below-middle 1,1.85,,,,G6,9/17 B-L,9/17 Below-left,Amp,5.18E-06,23.07,,,G6,9/21 BR,9/21 Below-right,Amp,1.62E-06,7.215710059,
21-Sep,Below-middle 2,0.631,,,,G7,9/14 C5,9/14 Cage-5,Amp,7.06E-06,31.45,,,G7,9/21 C5,9/21 Cage-5,Amp,2.03E-06,9.041908284,
21-Sep,Below-right,1.25,,,,G8,9/14 C5,9/14 Cage-5,Amp,5.67E-06,25.25,,,G8,9/21 C5,9/21 Cage-5,Amp,1.97E-06,8.774659764,
28-Sep,Below-left,5.15,,,,G9,9/14 C5,9/14 Cage-5,Amp,7.13E-06,31.76,,,G9,9/21 C5,9/21 Cage-5,Amp,1.32E-06,5.879467456,
28-Sep,Below-middle 1,3.65,,,,H1,9/17 A-R,9/17 Above-right,No Amp,0,0,,,H1,9/17 C2,9/17 Cage-2,Amp,5.50E-06,24.49778107,
28-Sep,Below-middle 2,4.41,,,,H2,9/17 A-R,9/17 Above-right,Amp,2.23E-06,9.93,,,H2,9/17 C2,9/17 Cage-2,Amp,6.22E-06,27.70476331,
28-Sep,Below-right,4.83,,,,H3,9/17 A-R,9/17 Above-right,No Amp,0,0,,,H3,9/17 C2,9/17 Cage-2,Amp,6.58E-06,29.30825444,
4-Oct,Below-left,5.22,,,,H4,9/17 BM-1,9/17 Below-middle 1,Amp,1.70E-05,75.72,,,H4,9/21 BM1,9/21 Below-middle 1,Amp,2.40E-06,10.68994083,
4-Oct,Below-middle 1,12.1,,,,H5,9/17 BM-1,9/17 Below-middle 1,Amp,1.65E-05,73.49,,,H5,9/21 BM1,9/21 Below-middle 1,Amp,3.35E-06,14.92137574,
4-Oct,Below-middle 2,2.45,,,,H6,9/17 BM-1,9/17 Below-middle 1,Amp,1.44E-05,64.14,,,H6,9/21 BM1,9/21 Below-middle 1,Amp,2.21E-06,9.843653847,
4-Oct,Below-right,9.33,,,,H7,NTC,Non-target control,No Amp,0,0,,,H7,NTC,Non-target control,No Amp,0,0,
12-Oct,Below-left,2.55,,,,H8,NTC,Non-target control,No Amp,0,0,,,H8,NTC,Non-target control,No Amp,0,0,
12-Oct,Below-middle 1,2.38,,,,H9,NTC,Non-target control,No Amp,0,0,,,H9,NTC,Non-target control,No Amp,0,0,
12-Oct,Below-middle 2,0.847,,,,,,,,,,,,,,,,,,
12-Oct,Below-right,1.69,,,,,,,,,,=,,,,,,,,
15-Oct,Below-left,1.11,,,,,,,,,,,,,,,,,,
15-Oct,Below-middle 1,0.693,,,,,,,,,,,,,,,,,,
15-Oct,Below-middle 2,0.285,,,,,,,,,,,,,,,,,,
15-Oct,Below-right,0.432,,,,,,,,,,,,,,,,,,
19-Oct,Below-left,1.01,,,,,,,,,,,,,,,,,,
19-Oct,Below-middle 1,<0.0005,,,,,,,,,,,,,,,,,,
19-Oct,Below-middle 2,<0.0005,,,,,,,,,,,,,,,,,,
19-Oct,Below-right,<0.0005,,,,,,,,,,,,,,,,,,
14-Sep,Cage-1,<0.0005,,,,,,,,,,,,,,,,,,
14-Sep,Cage-2,<0.0005,,,,,,,,,,,,,,,,,,
14-Sep,Cage-3,<0.0005,,,,,,,,,,,,,,,,,,
14-Sep,Cage-4,<0.0005,,,,,,,,,,,,,,,,,,
14-Sep,Cage-5,<0.0005,,,,,,,,,,,,,,,,,,
17-Sep,Cage-1,<0.0005,,,,,,,,,,,,,,,,,,
17-Sep,Cage-2,<0.0005,,,,,,,,,,,,,,,,,,
17-Sep,Cage-3,<0.0005,,,,,,,,,,,,,,,,,,
17-Sep,Cage-4,<0.0005,,,,,,,,,,,,,,,,,,
17-Sep,Cage-5,<0.0005,,,,,,,,,,,,,,,,,,
21-Sep,Cage-1,<0.0005,,,,,,,,,,,,,,,,,,
21-Sep,Cage-2,<0.0005,,,,,,,,,,,,,,,,,,
21-Sep,Cage-3,<0.0005,,,,,,,,,,,,,,,,,,
21-Sep,Cage-4,<0.0005,,,,,,,,,,,,,,,,,,
21-Sep,Cage-5,<0.0005,,,,,,,,,,,,,,,,,,
28-Sep,Cage-1,<0.0005,,,,,,,,,,,,,,,,,,
28-Sep,Cage-2,<0.0005,,,,,,,,,,,,,,,,,,
28-Sep,Cage-3,<0.0005,,,,,,,,,,,,,,,,,,
28-Sep,Cage-4,<0.0005,,,,,,,,,,,,,,,,,,
28-Sep,Cage-5,<0.0005,,,,,,,,,,,,,,,,,,
4-Oct,Cage-1,<0.0005,,,,,,,,,,,,,,,,,,
4-Oct,Cage-2,cage washed away in high flows,,,,,,,,,,,,,,,,,,
4-Oct,Cage-3,<0.0005,,,,,,,,,,,,,,,,,,
4-Oct,Cage-4,<0.0005,,,,,,,,,,,,,,,,,,
4-Oct,Cage-5,<0.0005,,,,,,,,,,,,,,,,,,
12-Oct,Cage-1,<0.0005,,,,,,,,,,,,,,,,,,
12-Oct,Cage-2,cage washed away in high flows,,,,,,,,,,,,,,,,,,
12-Oct,Cage-3,<0.0005,,,,,,,,,,,,,,,,,,
12-Oct,Cage-4,<0.0005,,,,,,,,,,,,,,,,,,
12-Oct,Cage-5,<0.0005,,,,,,,,,,,,,,,,,,
15-Oct,Cage-1,<0.0005,,,,,,,,,,,,,,,,,,
15-Oct,Cage-2,cage washed away in high flows,,,,,,,,,,,,,,,,,,
15-Oct,Cage-3,<0.0005,,,,,,,,,,,,,,,,,,
15-Oct,Cage-4,<0.0005,,,,,,,,,,,,,,,,,,
15-Oct,Cage-5,<0.0005,,,,,,,,,,,,,,,,,,
19-Oct,Cage-1,<0.0005,,,,,,,,,,,,,,,,,,
19-Oct,Cage-2,cage washed away in high flows,,,,,,,,,,,,,,,,,,
19-Oct,Cage-3,<0.0005,,,,,,,,,,,,,,,,,,
19-Oct,Cage-4,<0.0005,,,,,,,,,,,,,,,,,,
19-Oct,Cage-5,<0.0005,,,,,,,,,,,,,,,,,,