LOCUS Exported 10278 bp ds-DNA circular SYN 13-SEP-2019 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10278) AUTHORS waldripzachary TITLE Direct Submission JOURNAL Exported Friday, Sep 13, 2019 from SnapGene Viewer 4.3.11 https://www.snapgene.com FEATURES Location/Qualifiers source 1..10278 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 106..293 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" CDS complement(346..1020) /codon_start=1 /gene="S. cerevisiae TRP1" /product="phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis" /label=TRP1 /note="yeast auxotrophic marker" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA KK" promoter complement(1021..1122) /gene="S. cerevisiae TRP1" /label=TRP1 promoter primer_bind complement(1137..1153) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1539..2127) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2296..3111) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 3470..4634 /label=2u ori /note="yeast 2u plasmid origin of replication" promoter 4728..4746 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 4764..5032 /label=SNR52 promoter /note="promoter for the S. cerevisiae small nucleolar RNA gene SNR52" misc_RNA 5051..5126 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" terminator 5130..5149 /label=SUP4 terminator /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" misc_feature 5260..5602 /label=ADH1 Promoter misc_feature 5615..6064 /label=Protein A tag CDS 6083..10186 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 10199..10219 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 10223..10243 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 10247..10267 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" ORIGIN 1 caattgtcgt agatcttcgt cagcagagct tcaccattga agggctggcg gttggggtta 61 ttcgcaacgg cgactggctg tgaggatccg ccaagctaat tccgggcgaa tttcttatga 121 tttatgattt ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt 181 gactcttagg ttttaaaacg aaaattcttg ttcttgagta actctttcct gtaggtcagg 241 ttgctttctc aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgccg 301 gcaagtgcac aaacaatact taaataaata ctactcagta ataacctatt tcttagcatt 361 tttgacgaaa tttgctattt tgttagagtc ttttacacca tttgtctcca cacctccgct 421 tacatcaaca ccaataacgc catttaatct aagcgcatca ccaacatttt ctggcgtcag 481 tccaccagct aacataaaat gtaagctttc ggggctctct tgccttccaa cccagtcaga 541 aatcgagttc caatccaaaa gttcacctgt cccacctgct tctgaatcaa acaagggaat 601 aaacgaatga ggtttctgtg aagctgcact gagtagtatg ttgcagtctt ttggaaatac 661 gagtctttta ataactggca aaccgaggaa ctcttggtat tcttgccacg actcatctcc 721 atgcagttgg acgatatcaa tgccgtaatc attgaccaga gccaaaacat cctccttagg 781 ttgattacga aacacgccaa ccaagtattt cggagtgcct gaactatttt tatatgcttt 841 tacaagactt gaaattttcc ttgcaataac cgggtcaatt gttctctttc tattgggcac 901 acatataata cccagcaagt cagcatcgga atctagagca cattctgcgg cctctgtgct 961 ctgcaagccg caaactttca ccaatggacc agaactacct gtgaaattaa taacagacat 1021 actccaagct gcctttgtgt gcttaatcac gtatactcac gtgctcaata gtcaccaatg 1081 ccctccctct tggccctctc cttttctttt ttcgaccgaa ttaattcgta atcatggtca 1141 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 1201 agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt aattgcgttg 1261 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctggatta atgaatcggc 1321 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 1381 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 1441 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 1501 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 1561 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 1621 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 1681 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 1741 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 1801 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 1861 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 1921 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga 1981 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 2041 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 2101 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 2161 gctcagtgga acgaaaactc acgttaaggg attttggtca tgtggatgat ccagttcgat 2221 ttattcaaca aagccgccgt cccgtcaagt cagcgtaatg ctctgccagt gttacaacca 2281 attaaccaat tctgattaga aaaactcatc gagcatcaaa tgaaactgca atttattcat 2341 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc 2401 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc 2461 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc 2521 accatgagtg acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac 2581 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt 2641 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt 2701 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc 2761 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttgcctggga tcgcagtggt 2821 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa 2881 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt 2941 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc 3001 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt 3061 ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct 3121 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg 3181 tgcaatgtaa catcagagat tttgagacac aacgtggctt actaggatcg atccgacatg 3241 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat 3301 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 3361 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 3421 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccataa cgcatttaag 3481 cataaacacg cactatgccg ttcttctcat gtatatatat atacaggcaa cacgcagata 3541 taggtgcgac gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc 3601 tcgttttcgg aaacgctttg aagttcctat tccgaagttc ctattctcta gctagaaagt 3661 ataggaactt cagagcgctt ttgaaaacca aaagcgctct gaagacgcac tttcaaaaaa 3721 ccaaaaacgc accggactgt aacgagctac taaaatattg cgaataccgc ttccacaaac 3781 attgctcaaa agtatctctt tgctatatat ctctgtgcta tatccctata taacctaccc 3841 atccaccttt cgctccttga acttgcatct aaactcgacc tctacatttt ttatgtttat 3901 ctctagtatt actctttaga caaaaaaatt gtagtaagaa ctattcatag agtgaatcga 3961 aaacaatacg aaaatgtaaa catttcctat acgtagtata tagagacaaa atagaagaaa 4021 ccgttcataa ttttctgacc aatgaagaat catcaacgct atcactttct gttcacaaag 4081 tatgcgcaat ccacatcggt atagaatata atcggggatg cctttatctt gaaaaaatgc 4141 acccgcagct tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt tttttatgga 4201 agagaaaata gacaccaaag tagccttctt ctaaccttaa cggacctaca gtgcaaaaag 4261 ttatcaagag actgcattat agagcgcaca aaggagaaaa aaagtaatct aagatgcttt 4321 gttagaaaaa tagcgctctc gggatgcatt tttgtagaac aaaaaagaag tatagattct 4381 ttgttggtaa aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc agctcagatt 4441 ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa tgaagcacag 4501 attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc 4561 agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttctac aaaatgaagc 4621 acagatgctt cgttgcttgc atgcaacttc ttttcttttt ttttcttttc tctctccccc 4681 gttgttacta gtggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac 4741 taaagggaac aaaagctgga gcttctttga aaagataatg tatgattatg ctttcactca 4801 tatttataca gaaacttgat gttttctttc gagtatatac aaggtgatta catgtacgtt 4861 tgaagtacaa ctctagattt tgtagtgccc tcttgggcta gcggtaaagg tgcgcatttt 4921 ttcacaccct acaatgttct gttcaaaaga ttttggtcaa acgctgtaga agtgaaagtt 4981 ggtgcgcatg tttcggcgtt cgaaacttct ccgcagtgaa agataaatga tcgttggtag 5041 cactttgatg gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac 5101 ttgaaaaagt ggcaccgagt cggtggtgct ttttttgttt tttatgtctt cgagtcatgt 5161 aattagttat gtcacgctta cgttcacgcc ctccccccac atccgctcta accgaaaaac 5221 tagtctcacc atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta 5281 acgacaaaga cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa 5341 cacacgaaac tttttccttc cttcattcac gcacactact ctctaatgag caacggtata 5401 cggccttcct tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag 5461 tataaataga cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc 5521 ttccttgttt ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa 5581 gcttgaatta attccgggcg gagaattcga gctcatgcgt acgctgcagg tcgacggatc 5641 ccctcaacaa aacaaaaccg cggctcttgc gcaacacgat gaagccgtgg acaacaaatt 5701 caacaaagaa caacaaaacg cgttctatga gatcttacat ttacctaact taaacgaaga 5761 acaacgaaac gccttcatcc aaagtttaaa agatgaccca agccaaagcg ctaacctttt 5821 agcagaagct aaaaagctaa atgatgctca ggcgccgaaa gtagacaaca aattcaacaa 5881 agaacaacaa aacgcgttct atgagatctt acatttacct aacttaaacg aagaacaacg 5941 aaacgccttc atccaaagtt taaaagatga cccaagccaa agcgctaacc ttttagcaga 6001 agctaaaaag ctaaatgatg ctcaggcgcc gaaagtagac gcgaattccg cggctcttgc 6061 tagcgccccg ggttcaggtt caatggacaa gaagtattct atcggactgg ccatcgggac 6121 taatagcgtc gggtgggccg tgatcactga cgagtacaag gtgccctcta agaagttcaa 6181 ggtgctcggg aacaccgacc ggcattccat caagaaaaat ctgatcggag ctctcctctt 6241 tgattcaggg gagaccgctg aagcaacccg cctcaagcgg actgctagac ggcggtacac 6301 caggaggaag aaccggattt gttaccttca agagatattc tccaacgaaa tggcaaaggt 6361 cgacgacagc ttcttccata ggctggaaga atcattcctc gtggaagagg ataagaagca 6421 tgaacggcat cccatcttcg gtaatatcgt cgacgaggtg gcctatcacg agaaataccc 6481 aaccatctac catcttcgca aaaagctggt ggactcaacc gacaaggcag acctccggct 6541 tatctacctg gccctggccc acatgatcaa gttcagaggc cacttcctga tcgagggcga 6601 cctcaatcct gacaatagcg atgtggataa actgttcatc cagctggtgc agacttacaa 6661 ccagctcttt gaagagaacc ccatcaatgc aagcggagtc gatgccaagg ccattctgtc 6721 agcccggctg tcaaagagcc gcagacttga gaatcttatc gctcagctgc cgggtgaaaa 6781 gaaaaatgga ctgttcggga acctgattgc tctttcactt gggctgactc ccaatttcaa 6841 gtctaatttc gacctggcag aggatgccaa gctgcaactg tccaaggaca cctatgatga 6901 cgatctcgac aacctcctgg cccagatcgg tgaccaatac gccgaccttt tccttgctgc 6961 taagaatctt tctgacgcca tcctgctgtc tgacattctc cgcgtgaaca ctgaaatcac 7021 caaggcccct ctttcagctt caatgattaa gcggtatgat gagcaccacc aggacctgac 7081 cctgcttaag gcactcgtcc ggcagcagct tccggagaag tacaaggaaa tcttctttga 7141 ccagtcaaag aatggatacg ccggctacat cgacggaggt gcctcccaag aggaatttta 7201 taagtttatc aaacctatcc ttgagaagat ggacggcacc gaagagctcc tcgtgaaact 7261 gaatcgggag gatctgctgc ggaagcagcg cactttcgac aatgggagca ttccccacca 7321 gatccatctt ggggagcttc acgccatcct tcggcgccaa gaggacttct acccctttct 7381 taaggacaac agggagaaga ttgagaaaat tctcactttc cgcatcccct actacgtggg 7441 acccctcgcc agaggaaata gccggtttgc ttggatgacc agaaagtcag aagaaactat 7501 cactccctgg aacttcgaag aggtggtgga caagggagcc agcgctcagt cattcatcga 7561 acggatgact aacttcgata agaacctccc caatgagaag gtcctgccga aacattccct 7621 gctctacgag tactttaccg tgtacaacga gctgaccaag gtgaaatatg tcaccgaagg 7681 gatgaggaag cccgcattcc tgtcaggcga acaaaagaag gcaattgtgg accttctgtt 7741 caagaccaat agaaaggtga ccgtgaagca gctgaaggag gactatttca agaaaattga 7801 atgcttcgac tctgtggaga ttagcggggt cgaagatcgg ttcaacgcaa gcctgggtac 7861 ctaccatgat ctgcttaaga tcatcaagga caaggatttt ctggacaatg aggagaacga 7921 ggacatcctt gaggacattg tcctgactct cactctgttc gaggaccggg aaatgatcga 7981 ggagaggctt aagacctacg cccatctgtt cgacgataaa gtgatgaagc aacttaaacg 8041 gagaagatat accggatggg gacgccttag ccgcaaactc atcaacggaa tccgggacaa 8101 acagagcgga aagaccattc ttgatttcct taagagcgac ggattcgcta atcgcaactt 8161 catgcaactt atccatgatg attccctgac ctttaaggag gacatccaga aggcccaagt 8221 gtctggacaa ggtgactcac tgcacgagca tatcgcaaat ctggctggtt cacccgctat 8281 taagaagggt attctccaga ccgtgaaagt cgtggacgag ctggtcaagg tgatgggtcg 8341 ccataaacca gagaacattg tcatcgagat ggccagggaa aaccagacta cccagaaggg 8401 acagaagaac agcagggagc ggatgaaaag aattgaggaa gggattaagg agctcgggtc 8461 acagatcctt aaagagcacc cggtggaaaa cacccagctt cagaatgaga agctctatct 8521 gtactacctt caaaatggac gcgatatgta tgtggaccaa gagcttgata tcaacaggct 8581 ctcagactac gacgtggacg ccatcgtccc tcagagcttc ctcaaagacg actcaattga 8641 caataaggtg ctgactcgct cagacaagaa ccggggaaag tcagataacg tgccctcaga 8701 ggaagtcgtg aaaaagatga agaactattg gcgccagctt ctgaacgcaa agctgatcac 8761 tcagcggaag ttcgacaatc tcactaaggc tgagaggggc ggactgagcg aactggacaa 8821 agcaggattc attaaacggc aacttgtgga gactcggcag attactaaac atgtcgccca 8881 aatccttgac tcacgcatga ataccaagta cgacgaaaac gacaaactta tccgcgaggt 8941 gaaggtgatt accctgaagt ccaagctggt cagcgatttc agaaaggact ttcaattcta 9001 caaagtgcgg gagatcaata actatcatca tgctcatgac gcatatctga atgccgtggt 9061 gggaaccgcc ctgatcaaga agtacccaaa gctggaaagc gagttcgtgt acggagacta 9121 caaggtctac gacgtgcgca agatgattgc caaatctgag caggagatcg gaaaggccac 9181 cgcaaagtac ttcttctaca gcaacatcat gaatttcttc aagaccgaaa tcacccttgc 9241 aaacggtgag atccggaaga ggccgctcat cgagactaat ggggagactg gcgaaatcgt 9301 gtgggacaag ggcagagatt tcgctaccgt gcgcaaagtg ctttctatgc ctcaagtgaa 9361 catcgtgaag aaaaccgagg tgcaaaccgg aggcttttct aaggaatcaa tcctccccaa 9421 gcgcaactcc gacaagctca ttgcaaggaa gaaggattgg gaccctaaga agtacggcgg 9481 attcgattca ccaactgtgg cttattctgt cctggtcgtg gctaaggtgg aaaaaggaaa 9541 gtctaagaag ctcaagagcg tgaaggaact gctgggtatc accattatgg agcgcagctc 9601 cttcgagaag aacccaattg actttctcga agccaaaggt tacaaggaag tcaagaagga 9661 ccttatcatc aagctcccaa agtatagcct gttcgaactg gagaatgggc ggaagcggat 9721 gctcgcctcc gctggcgaac ttcagaaggg taatgagctg gctctcccct ccaagtacgt 9781 gaatttcctc taccttgcaa gccattacga gaagctgaag gggagccccg aggacaacga 9841 gcaaaagcaa ctgtttgtgg agcagcataa gcattatctg gacgagatca ttgagcagat 9901 ttccgagttt tctaaacgcg tcattctcgc tgatgccaac ctcgataaag tccttagcgc 9961 atacaataag cacagagaca aaccaattcg ggagcaggct gagaatatca tccacctgtt 10021 caccctcacc aatcttggtg cccctgccgc attcaagtac ttcgacacca ccatcgaccg 10081 gaaacgctat acctccacca aagaagtgct ggacgccacc ctcatccacc agagcatcac 10141 cggactttac gaaactcgga ttgacctctc acagctcgga ggggatgagg gagctgatcc 10201 aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtagatccaa aaaagaagag 10261 aaaggtatag gtttaaac //