Field Name(s),Field Description
run_location,the alphanumeric letterings used to identify the sample’s qPCR well
run_platform,the qPCR platform that generated the raw qPCR output
threshold,the pre-set fluorescence cut-off level used to calculate threshold cycle (Ct) value
primer_seq,the complementary DNA sequence known as oligonucleotides to the target DNA region
target_gene,the target DNA sequence for PCR amplification 
basisofRecord ,"The type of the sample record (e.g. ""environmental sample"", ""living specimen"")"
recordedBy,Name(s) of sample collector(s) 
establishmentMeans,"The process by which the target species occurs within a region. (e.g. ""invasive"", ""native"") "
associatedTaxa,Identifier(s) or name(s) of the taxa that co-occur with the target species. 
organismScope,"The target species which can be a discrete organism or a specific kind of organism aggregation (e.g. “virus”, “multicellular organism”)"
eventDate,The collection date of the biological sample
eventRemarks,Extra note about the sampling event 
continent,The name of the continent in which the sample is collected.
country,The name of the country in which the sample is collected.
stateProvince,"The name of the province (or state, region, etc.) in which the sample is collected. "
locality,"The detailed description of the location in which the sample is collected. (e.g. ""Guelph, 20 km NE via Highway 6"")"
minimumElevationInMeters,The lower limit of the range of altitude (in meters; above sea level) of the sampling event. 
maximumElevationInMeters,The upper limit of the range of altitude (in meters; above sea level) of the sampling event. 
minimumDepthInMeters,The lower limit of the range of sampling depth (in meters) below the surface. 
maximumDepthInMeters,The upper limit of the range of sampling depth (in meters) below the surface. 
verbatimElevation,A description of the elevation of the sampling location （in meters)
verbatimDepth,A description of the depth of the sampling location (in meters)
decimalLatitude ,the biological sample collection GPS latitude (in decimal degrees; Geodetic Datum: EPSG:3857) 
decimalLongtitude,the biological sample collection GPS longitude (in decimal degrees; Geodetic Datum: EPSG:3857) 
coordinateUncertaintyInMeters,The distance (in meters) from decimalLatitude and decimalLongitude. This is the information providing the smallest circular area where the whole sampling event occurs. 
taxonID,the unique identifier for the species target of the qPCR assay
kingdom,The name of the kingdom in which the target species is classified.
phylum,The name of the phylum in which the target species is classified.
class,The name of the class in which the target species is classified.
order,The name of the order in which the target species is classified.
family,The name of the family in which the target species is classified.
genus,The name of the genus in which the target species is classified.
Species,The name of the species in which the  target species is classified. 
vernacularName,The common name (if any) of the target species.