,,,,,,,,,, GROUP = ANTHRONE ASSAY,,,,,,,,,, ,,,,,,,,,, "Case Summariesa,b",,,,,,,,,, TEST,,CONTROL ( DISTILLED WATER),SeNP,CaOH,CHX,NaOCl,% Reduction of SeNP from CONTROL,% Reduction of CaOH from CONTROL,% Reduction of CHX from CONTROL,% Reduction of NaOCl from CONTROL 1,,31.02,8.4,28.28,17.25,10.23,72.92,8.83,44.39,67.02 2,,30.92,8.6,28.23,17.36,10.16,72.32,8.7,43.86,67.14 3,,32.05,8.2,28.13,17.19,10.31,74.54,12.23,46.37,67.83 Total,Mean,31.33,8.373,28.2133,17.2667,10.2333,73.2587,9.9213,44.8703,67.3313 ,Std. Deviation,0.62554,0.2013,0.07638,0.08622,0.07506,1.14995,2.00131,1.32191,0.43734 ,,,,,,,,,, ,,,,,,,,,, "The mean percentage reduction of Carbohydrates contents in biofilm compared to control was highest in ( SeNP(73%), followed in NaOCl( 67.3% , in CHX (44.87% and lowest was in CaOH (9.92 %). The reduction was highly significant (p<.001) in all biofilm( SeNP,CaOH,CHX and NaOCl) compared to CONTROL (DISTILLED WATER) under Anthrone Assay Group.",,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, GROUP = BRADFORD ASSAY,,,,,,,,,, ,,,,,,,,,, "Case Summariesa,b",,,,,,,,,, TEST,,CONTROL ( DISTILLED WATER),SeNP,CaOH,CHX,NaOCl,% Reduction of SeNP from CONTROL,% Reduction of CaOH from CONTROL,% Reduction of CHX from CONTROL,% Reduction of NaOCl from CONTROL 1,,17.92,5,16.14,10.42,8.52,71.99,9.93,41.85,52.46 2,,18.23,5.2,16.21,10.35,8.56,71.42,11.08,43.23,53.04 3,,17.82,5.4,15.82,10.12,8.48,69.92,11.22,43.21,52.41 Total,Mean,17.99,5.197,16.0567,10.2967,8.52,71.1096,10.7457,42.7627,52.6376 ,Std. Deviation,0.21378,0.1704,0.20793,0.15695,0.04,1.06716,0.70737,0.78811,0.35296 a. GROUP = BRADFORD ASSAY,,,,,,,,,, ,,,,,,,,,, "The mean percentage reduction of Protein contents in biofilm compared to control was highest in ( SeNP(71%), followed in NaOCl(52%) , in CHX (43% )and lowest was in CaOH (10.7%). The reduction of protein contents was highly significant (p<.001) in all biofilm( SeNP,CaOH,CHX and NaOCl) compared to CONTROL (DISTILLED WATER) under Bradford Assay Group.",,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, GROUP = ANTIBIOFILM INHIBITION,,,,,,,,,, ,,,,,,,,,, "Case Summariesa,b",,,,,,,,,, TEST,,CONTROL ( DISTILLED WATER),SeNP,CaOH,CHX,NaOCl,% Reduction of SeNP from CONTROL,% Reduction of CaOH from CONTROL,% Reduction of CHX from CONTROL,% Reduction of NaOCl from CONTROL 1,,1.32,64.2,17.25,59.38,56.24,-4765.91,-1206.82,-4398.48,-4160.61 2,,1.45,66.4,19.63,58.73,57.63,-4475.86,-1253.79,-3950.34,-3874.48 3,,1.38,65.9,19.72,57.64,56.32,-4674.64,-1328.99,-4076.81,-3981.16 Total,Mean,1.3833,65.49,18.8667,58.5833,56.73,-4638.8029,-1263.1989,-4141.8804,-4005.4161 ,Std. Deviation,0.06506,1.1152,1.4008,0.87922,0.78045,148.30683,61.62439,231.04725,144.59573 ,,,,,,,,,, ,,,,,,,,,, "The mean percentage increase in growth of biofilm compared to control was highest in ( SeNP(4638%%), followed in CHX(4141%) in NaOCl(4005%) and lowest was in CaOH (1263%). %). The increase in growth was highly significant (p<.001) in all biofilm( SeNP,CaOH,CHX and NaOCl) compared to CONTROL (DISTILLED WATER) under Antibiofilm inhibition Group.",,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, Group: Disk diffusion assay,,,,,,,,,, ,,,,,,,,,, Sl. No,Test Conditions,Concentrations,Zone of Inhibition (mm),-2,-3,,,,, ,,,-1,,,,,,, 1,Control ,----,----,,,,,,, ,,10 μl,11,12,11,,,,, 2,SeNP (1mg/ml),20 μl,17,16,16.5,,,,, ,,30 μl,22,21,20,,,,, ,,40 μl,29,28,28.5,,,,, 3,,,,,,,,,, ,Ca(OH)₂(1mg/ml),20 μl,7,6.5,7,,,,, ,,,,,,,,,, 4,CHX,20 μl,13,12,14,,,,, 5,NaOCl,20 μl,15,14,15,,,,, 6,Guava leaf extract,20 μl,--,--,--,,,,, 7,Sodium selenite (25 mM),20 μl,--,--,--,,,,, ,,,,,,,,,, ,,,,,,,,,, NPar Tests,,,,,,,,,, ,,,,,,,,,, Descriptive Statistics,,,,,,,,,, ,N,Mean,Std. Deviation,Minimum,Maximum,Percentiles,,,, ,,,,,,25th,50th (Median),75th,, Control(20?l),3,0,0,0,0,0,0,0,, SeNP(1mg/dl) (Mean(10.20.30.40),3,19.3333,0.38188,19,19.75,19,19.25,19.75,, SeNP (1mg/ml)10?l,3,11.33,0.577,11,12,11,11,12,, SeNP (1mg/ml)20?l,3,16.5,0.5,16,17,16,16.5,17,, SeNP (1mg/ml)30?l,3,21,1,20,22,20,21,22,, SeNP (1mg/ml)40?l,3,28.5,0.5,28,29,28,28.5,29,, CaOH (20?l),3,6.83,0.289,6,7,6.5,7,7,, CHX(20?l),3,13,1,12,14,12,13,14,, NaOCl(20?l),3,14.67,0.577,14,15,14,15,15,, ,,,,,,,,,, ,,,,,,,,,, Wilcoxon Signed Ranks Test,,,,,,,,,, ,,,,,,,,,, Ranks,,,,,,,,,, ,,N,Mean Rank,Sum of Ranks,,,,,, SeNP (1mg/ml)10?l - Control(20?l),Negative Ranks,0a,0,0,,,,,, ,Positive Ranks,3b,2,6,,,,,, ,Ties,0c,,,,,,,, ,Total,3,,,,,,,, SeNP (1mg/ml)20?l - Control(20?l),Negative Ranks,0d,0,0,,,,,, ,Positive Ranks,3e,2,6,,,,,, ,Ties,0f,,,,,,,, ,Total,3,,,,,,,, SeNP (1mg/ml)30?l - Control(20?l),Negative Ranks,0g,0,0,,,,,, ,Positive Ranks,3h,2,6,,,,,, ,Ties,0i,,,,,,,, ,Total,3,,,,,,,, SeNP(1mg/dl) (Mean(10.20.30.40) - Control(20?l),Negative Ranks,0j,0,0,,,,,, ,Positive Ranks,3k,2,6,,,,,, ,Ties,0l,,,,,,,, ,Total,3,,,,,,,, SeNP (1mg/ml)40?l - SeNP(1mg/dl) (Mean(10.20.30.40),Negative Ranks,0m,0,0,,,,,, ,Positive Ranks,3n,2,6,,,,,, ,Ties,0o,,,,,,,, ,Total,3,,,,,,,, CaOH (20?l) - Control(20?l),Negative Ranks,0p,0,0,,,,,, ,Positive Ranks,3q,2,6,,,,,, ,Ties,0r,,,,,,,, ,Total,3,,,,,,,, CHX(20?l) - Control(20?l),Negative Ranks,0s,0,0,,,,,, ,Positive Ranks,3t,2,6,,,,,, ,Ties,0u,,,,,,,, ,Total,3,,,,,,,, NaOCl(20?l) - Control(20?l),Negative Ranks,0v,0,0,,,,,, ,Positive Ranks,3w,2,6,,,,,, ,Ties,0x,,,,,,,, ,Total,3,,,,,,,, a. SeNP (1mg/ml)10?l < Control(20?l),,,,,,,,,, b. SeNP (1mg/ml)10?l > Control(20?l),,,,,,,,,, c. SeNP (1mg/ml)10?l = Control(20?l),,,,,,,,,, d. SeNP (1mg/ml)20?l < Control(20?l),,,,,,,,,, e. SeNP (1mg/ml)20?l > Control(20?l),,,,,,,,,, f. SeNP (1mg/ml)20?l = Control(20?l),,,,,,,,,, g. SeNP (1mg/ml)30?l < Control(20?l),,,,,,,,,, h. SeNP (1mg/ml)30?l > Control(20?l),,,,,,,,,, i. SeNP (1mg/ml)30?l = Control(20?l),,,,,,,,,, j. SeNP(1mg/dl) (Mean(10.20.30.40) < Control(20?l),,,,,,,,,, k. SeNP(1mg/dl) (Mean(10.20.30.40) > Control(20?l),,,,,,,,,, l. SeNP(1mg/dl) (Mean(10.20.30.40) = Control(20?l),,,,,,,,,, m. SeNP (1mg/ml)40?l < SeNP(1mg/dl) (Mean(10.20.30.40),,,,,,,,,, n. SeNP (1mg/ml)40?l > SeNP(1mg/dl) (Mean(10.20.30.40),,,,,,,,,, o. SeNP (1mg/ml)40?l = SeNP(1mg/dl) (Mean(10.20.30.40),,,,,,,,,, p. CaOH (20?l) < Control(20?l),,,,,,,,,, q. CaOH (20?l) > Control(20?l),,,,,,,,,, r. CaOH (20?l) = Control(20?l),,,,,,,,,, s. CHX(20?l) < Control(20?l),,,,,,,,,, t. CHX(20?l) > Control(20?l),,,,,,,,,, u. CHX(20?l) = Control(20?l),,,,,,,,,, v. NaOCl(20?l) < Control(20?l),,,,,,,,,, w. NaOCl(20?l) > Control(20?l),,,,,,,,,, x. NaOCl(20?l) = Control(20?l),,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, Test Statisticsb,,,,,,,,,, ,SeNP (1mg/ml)10?l - Control(20?l),SeNP (1mg/ml)20?l - Control(20?l),SeNP (1mg/ml)30?l - Control(20?l),SeNP(1mg/dl) (Mean(10.20.30.40) - Control(20?l),SeNP (1mg/ml)40?l - SeNP(1mg/dl) (Mean(10.20.30.40),CaOH (20?l) - Control(20?l),CHX(20?l) - Control(20?l),NaOCl(20?l) - Control(20?l),, Z,-1.633a,-1.604a,-1.604a,-1.604a,-1.604a,-1.633a,-1.604a,-1.633a,, Asymp. Sig. (2-tailed),0.102,0.109,0.109,0.109,0.109,0.102,0.109,0.102,, a. Based on negative ranks.,,,,,,,,,, b. Wilcoxon Signed Ranks Test,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, T-Test,,,,,,,,,, ,,,,,,,,,, Paired Samples Statistics,,,,,,,,,, ,,Mean,N,Std. Deviation,Std. Error Mean,,,,, Pair 1,Control(20?l),0,3,0,0,,,,, ,SeNP (1mg/ml)10?l,11.33,3,0.577,0.333,,,,, Pair 2,Control(20?l),0,3,0,0,,,,, ,SeNP (1mg/ml)20?l,16.5,3,0.5,0.289,,,,, Pair 3,Control(20?l),0,3,0,0,,,,, ,SeNP (1mg/ml)30?l,21,3,1,0.577,,,,, Pair 4,Control(20?l),0,3,0,0,,,,, ,SeNP (1mg/ml)40?l,28.5,3,0.5,0.289,,,,, Pair 5,Control(20?l),0,3,0,0,,,,, ,SeNP(1mg/dl) (Mean(10.20.30.40),19.3333,3,0.38188,0.22048,,,,, Pair 6,Control(20?l),0,3,0,0,,,,, ,CaOH (20?l),6.83,3,0.289,0.167,,,,, Pair 7,Control(20?l),0,3,0,0,,,,, ,CHX(20?l),13,3,1,0.577,,,,, Pair 8,Control(20?l),0,3,0,0,,,,, ,NaOCl(20?l),14.67,3,0.577,0.333,,,,, ,,,,,,,,,, ,,,,,,,,,, Paired Samples Test,,,,,,,,,, ,,Paired Differences,,,,,t,df,Sig. (2-tailed), ,,Mean,Std. Deviation,Std. Error Mean,95% Confidence Interval of the Difference,,,,, ,,,,,Lower,Upper,,,, Pair 1,Control(20?l) - SeNP (1mg/ml)10?l,-11.333,0.577,0.333,-12.768,-9.899,-34,2,0.001, Pair 2,Control(20?l) - SeNP (1mg/ml)20?l,-16.5,0.5,0.289,-17.742,-15.258,-57.158,2,0, Pair 3,Control(20?l) - SeNP (1mg/ml)30?l,-21,1,0.577,-23.484,-18.516,-36.373,2,0.001, Pair 4,Control(20?l) - SeNP (1mg/ml)40?l,-28.5,0.5,0.289,-29.742,-27.258,-98.727,2,0, Pair 5,Control(20?l) - SeNP(1mg/dl) (Mean(10.20.30.40),-1.93E+01,0.38188,0.22048,-20.28198,-18.38469,-87.688,2,0, Pair 6,Control(20?l) - CaOH (20?l),-6.833,0.289,0.167,-7.55,-6.116,-41,2,0.001, Pair 7,Control(20?l) - CHX(20?l),-13,1,0.577,-15.484,-10.516,-22.517,2,0.002, Pair 8,Control(20?l) - NaOCl(20?l),-14.667,0.577,0.333,-16.101,-13.232,-44,2,0.001, ,,,,,,,,,, ,,,,,,,,,, Summarize,,,,,,,,,, ,,,,,,,,,, Case Summaries,,,,,,,,,, ,Control(20?l),SeNP (1mg/ml)10?l,SeNP (1mg/ml)20?l,SeNP (1mg/ml)30?l,SeNP (1mg/ml)40?l,SeNP(1mg/dl) (Mean(10.20.30.40),CaOH (20?l),CHX(20?l),NaOCl(20?l), Mean,0,11.33,16.5,21,28.5,19.3333,6.83,13,14.67, Std. Deviation,0,0.577,0.5,1,0.5,0.38188,0.289,1,0.577, ,,,,,,,,,, " The mean Zone of Inhibition(mm) was lowest in CaOH (6.8), and followed by CHX(13), NaOCl(14.7) and higher in all concentration of SeNP’s (10/20/30/and 40) and also higher in means of SeNP’s in different concentration(19.3) compared to other different test conditions. ",,,,,,,,,, The differences of mean zone of inhibition under different treatment conditions was significantly different (p<.01) against Control (Enterococcus faecalis). ,,,,,,,,,, ,,,,,,,,,, ,Viable Cell Percentage (%),,,,,,,,, Control,Replicate 1,Replicate 2,Replicate 3,Average,St.Dev,,,,, 0 Hrs,47.1,48.2,46.1,47.13333,1.050397,,,,, 24hrs,89.2,88.1,89.9,89.06667,0.907377,,,,, 48hrs,96.3,96.7,95.5,96.16667,0.61101,,,,, ,,,,,,,,,, SeNP,,,,,,,,,, 0 Hrs,45.5,45.9,44.1,45.16667,0.945163,,,,, 24hrs,21.01,21.05,22.1,21.38667,0.618088,,,,, 48hrs,11.8,12,12.6,12.13333,0.416333,,,,, ,,,,,,,,,, Ca(OH)2,,,,,,,,,, 0 Hrs,48.4,48.2,47.4,48,0.52915,,,,, 24hrs,72.3,72.5,71.8,72.2,0.360555,,,,, 48hrs,57.8,57.9,58.6,58.1,0.43589,,,,, ,,,,,,,,,, CHX,,,,,,,,,, 0 Hrs,47.3,47.5,46.5,47.1,0.52915,,,,, 24hrs,29.5,29.9,30.7,30.03333,0.61101,,,,, 48hrs,18.68,18.93,19.84,19.15,0.610492,,,,, ,,,,,,,,,, NaOCl,,,,,,,,,, 0 Hrs,48.4,49.23,49.54,49.05667,0.589435,,,,, 24hrs,27.35,27.12,26.81,27.09333,0.270986,,,,, 48hrs,17.24,16.85,16.92,17.00333,0.207926,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,,