Supplementary material,,,,,,,,,, Enhanced soluble sugar content in tomato fruit using CRISPR/Cas9-mediated SlINVINH1 and SlVPE5 gene editing,,,,,,,,,, "Baike Wang1, Ning Li1,2, Shaoyong Huang2, Jiahui Hu1,3, Qiang Wang1,2, Yaping Tang1, Tao Yang1, Patiguli Asmutola1, Juan Wang1,*, Qinghui Yu1,**",,,,,,,,,, "1 Institute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, China, 2 College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi, China, 3 Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi, China",,,,,,,,,, ,,,,,,,,,, "*Corresponding author: Juan Wang, E-mail: 278089648@qq.com",,,,,,,,,, "**Corresponding author: Qinghui Yu, E-mail: yuqinghui98@sina.com",,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, Supplementary Table 1. Primers for detection of target region,,,,,,,,,, Target locus,,,Sequence,,,,,,, SlINVINH1 Target1-2 Forward,,,GACTATCTCCAGGTCCAAACA,,,,,,, SlINVINH1 Target1-2 Reverse,,,TTCACTAAGCAACCAGTAAGA,,,,,,, SlVPE5 Target1 Forward,,,GCGTAAGATAGCACGAAGGGAT,,,,,,, SlVPE5 Target1 Reverse,,,AAACGAACTAGATCGGAAGAA,,,,,,, SlVPE5 Target2 Forward,,,AGAATGGAATAAACCGGCTCT,,,,,,, SlVPE5 Target2 Reverse,,,CATCTCCCGTGTAATCCTGA,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, Supplementary Table 2. Detected zygosity of T0 independent transgenic lines of SlINVINH1 and SlVPE5,,,,,,,,,, Target gene,No. of plants examined,Zygosity,,,,,,,, ,,Homozygote,Biallele,Heterozygote,Chimera,WT,,,, SlINVINH1,14,1(7.14%),2(14.29%),1(7.14%),2(14.29%),8(57.14%),,,, SlVPE5,13,1(7.69%),1(7.69%),5(38.46%),1(7.69%),5(38.46%),,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, Supplementary Table 3. The content of soluble sugar and TSS in the red fruit of T0 mutant lines,,,,,,,,,, Target genes,Lines,Glucose,,,Fructose,,,TSS,, ,,mg/g fw,,,mg/g fw,,,Brix %,, SlINVINH1,,Rep,Avg,P-value,Rep,Avg,P-value,Rep,Avg,P-value ,WT,9.9,10.53¡À0.71,,9.6,9.9¡À0.30,,3.9,3.87¡À0.15, ,,11.3,,,10.2,,,3.7,, ,,10.4,,,9.9,,,4,, ,INH-1,14.2,14.77¡À0.55,0,13.9,14.1¡À0.20,0,5.1,5.1¡À0.20,0.001 ,,14.8,,,14.1,,,4.9,, ,,15.3,,,14.3,,,5.3,, ,INH-2,13.9,14.37¡À0.42,0,13.8,13.43¡À0.40,0,4.8,5.03¡À0.25,0.002 ,,14.5,,,13,,,5.3,, ,,14.7,,,13.5,,,5,, ,INH-3,11.1,10.63¡À0.57,0.998,10.1,9.6¡À0.43,0.743,3.9,3.77¡À0.15,0.966 ,,10,,,9.4,,,3.8,, ,,10.8,,,9.3,,,3.6,, ,INH-4,14.4,14.83¡À0.40,0,13.9,14.13¡À0.32,0,4.7,5.13¡À0.40,0.001 ,,14.9,,,14.5,,,5.2,, ,,15.2,,,14,,,5.5,, SlVPE5,WT,10.2,10.7¡À0.62,,9.8,10¡À0.62,,3.7,3.7¡À0.30, ,,11.4,,,10.7,,,4,, ,,10.5,,,9.5,,,3.4,, ,VPE-1,14.5,14.47¡À0.45,0,14.3,13.73¡À0.55,0,4.6,4.83¡À0.21,0.003 ,,14.9,,,13.2,,,4.9,, ,,14,,,13.7,,,5,, ,VPE-2,10.4,10.77¡À0.32,0.99,9.9,10.2¡À0.26,0.937,3.5,3.83¡À0.31,0.83 ,,10.9,,,10.4,,,4.1,, ,,11,,,10.3,,,3.9,, ,VPE-7,13.9,14.53¡À0.60,0,14.2,14.3¡À0.56,0,5,4.9¡À0.26,0.002 ,,15.1,,,14.9,,,5.1,, ,,14.6,,,13.8,,,4.6,, "Note: WT=Wild-type, fw=fruit weight, Rep=Three test replicates, Avg=Average.",,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, Supplementary Table 4. The content of soluble sugar and TSS in the red fruit of F2 lines,,,,,,,,,, Lines,Glucose,,,Fructose,,,TSS,,, ,mg/g fw,,,mg/g fw,,,Brix %,,, ,Rep,Avg,P-value,Rep,Avg,P-value,Rep,Avg,P-value, WT,9.5,10.43¡À1.14,,8.9,9.6¡À0.61,,3.9,3.86¡À0.15,, ,11.7,,,9.9,,,3.7,,, ,10.1,,,10,,,4,,, F2-1,16.1,17.2¡À1.10,0,15.1,16.17¡À1.05,0.001,5.5,6¡À0.46,0.011, ,18.3,,,16.2,,,6.4,,, ,17.2,,,17.2,,,6.1,,, F2-2,17.3,17.47¡À0.57,0,16.2,16.27¡À1.40,0.001,6.2,6.27¡À0.70,0.007, ,18.1,,,14.9,,,5.6,,, ,17,,,17.7,,,7,,, "Note: WT=Wild-type, fw=fruit weight, Rep=Three test replicates, Avg=Average.",,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, Supplementary Table 5. Comparison of soluble sugar and TSS contents in the red fruit between T0 and F2 lines,,,,,,,,,, Genotype of lines,Lines,Glucose,,,Fructose,,,TSS,, ,,mg/g fw,,,mg/g fw,,,Brix %,, T0,,Rep,Avg,P-value,Rep,Avg,P-value,Rep,Avg,P-value ,INH-4,14.4,14.68¡À0.49,,13.9,14.22¡À0.42,,4.7,5.02¡À0.33, ,,14.9,,,14.5,,,5.2,, ,,15.2,,,14,,,5.5,, ,VPE-7,13.9,,,14.2,,,5,, ,,15.1,,,14.9,,,5.1,, ,,14.6,,,13.8,,,4.6,, F2,F2-1,16.1,17.33¡À0.80,0,15.1,16.22¡À1.11,0,5.5,6.13¡À0.55,0 ,,18.3,,,16.2,,,6.4,, ,,17.2,,,17.2,,,6.1,, ,F2-2,17.3,,,16.2,,,6.2,, ,,18.1,,,14.9,,,5.6,, ,,17,,,17.7,,,7,, "Note: fw=fruit weight, Rep=Three test replicates, Avg=Average.",,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,, ,,,,,,,,,,