FastQCFastQC Report
Mon 16 Oct 2017
E743-IP-1-mRNA_all.fq.clean_fq_reverse

Summary

[OK] Basic Statistics

Measure Value
Filename E743-IP-1-mRNA_all.fq.clean_fq_reverse
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 29540199
Sequence length 16-147
%GC 51

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAG 300703 1.0179450720694196 No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG 170698 0.5778498648570377 No Hit
AGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCTTGGTG 137193 0.4644281509410279 No Hit
AATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTG 136651 0.46259336303049275 No Hit
CCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAG 127848 0.43279329296325997 No Hit
ACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGA 105589 0.3574417355820792 No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGGGGAGG 94578 0.32016710517082164 No Hit
TGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCTTG 88963 0.301159108643784 No Hit
CAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACA 87064 0.29473058052181705 No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA 84844 0.28721539756722697 No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG 84589 0.2863521670927132 No Hit
GGCCCTTGAAAATCCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTAC 83419 0.2823914625625914 No Hit
CCTTGAAAATCCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCA 82123 0.2780042206215334 No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG 72444 0.24523869998302994 No Hit
TTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTC 71585 0.24233079811006014 No Hit
AGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTA 70540 0.2387932457733274 No Hit
TGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTG 66337 0.22456517642281287 No Hit
TAGTGACGCGCATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACT 64166 0.21721586912803126 No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA 60669 0.20537776336577826 No Hit
GGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTA 58693 0.19868857349268362 No Hit
TGGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGT 55444 0.18769000168211458 No Hit
GGAGCCCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGA 49620 0.16797449468773043 No Hit
GCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGG 48618 0.16458250670552355 No Hit
TGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCG 47647 0.16129546046727714 No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAC 47461 0.1606658100035142 No Hit
TGGCCCTTGAAAATCCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTA 44951 0.15216891396026141 No Hit
AATTAGTGACGCGCATGAATGGATGAACGAGATTCCCACTGTCCCTACCT 43852 0.14844855987598457 No Hit
CGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCT 39871 0.13497200882092905 No Hit
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTG 39121 0.13243309566059455 No Hit
GTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGC 36663 0.12411223092979164 No Hit
CACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGC 35707 0.12087596295475193 No Hit
TATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAA 35297 0.11948802376043574 No Hit
ACGGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCC 33250 0.11255848344149612 No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAG 33169 0.11228428082018 No Hit
CCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCTTGGTGGT 32923 0.11145151730359026 No Hit
ACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGT 32115 0.10871626152552324 No Hit
GCATTGAATGAATTTAAATCATCTTCTTTGGCAGTAATCCATCTTTGACT 31998 0.10832019107251106 No Hit
AAATCCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCATATCCG 31865 0.10786995713874506 No Hit
TTGGCAGTAATCCATCTTTGACTTAAGGCAATACAAGCATTTGTCAGAGT 31191 0.10558832051199114 No Hit
GGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGT 30703 0.10393633434900015 No Hit
GCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAA 30673 0.10383477782258677 No Hit
CAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACG 30517 0.10330668388523719 No Hit
CCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCG 30085 0.10184426990488453 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
ACTTT 6053145 3.0491333 7.1566014 25-29
TTTTT 4380250 2.8594983 10.703882 130-134
CTTTG 5961645 2.8492718 7.0552387 25-29
CGCCG 8487850 2.8288815 5.9048967 4
TGAAC 8778520 2.7532961 9.331817 70-74
TTCCC 4546390 2.564927 5.5891185 7
TTTTC 4024690 2.502625 9.332668 130-134
TTGAA 7564665 2.4908574 6.4981422 30-34
TTGGT 6300680 2.4298928 5.9801545 45-49
GTTTT 4817655 2.4173024 6.554484 120-124
AACTT 5698420 2.3253145 7.5456753 25-29
TTTTG 4609735 2.3129764 6.0446534 2
CTGGC 6277740 2.1965847 6.0255456 85-89
GAACT 6984620 2.1906571 5.9083657 25-29
CTTTT 3486235 2.1678042 5.2908425 70-74
GTTCC 4746530 2.1608093 6.449161 100-104
TCCCT 3718640 2.097937 6.134984 8
TATTC 4064530 2.0474138 15.506858 3
CCCTC 3747405 2.0137773 8.722858 140-142
GGCGC 7485770 2.0131934 5.3841786 90-94
GCCGC 5963195 1.9874493 5.53827 25-29
TGTTG 5102335 1.9677442 7.019414 5
TGGTT 5099335 1.9665872 5.108695 8
TGGTG 6607160 1.9584786 9.457594 1
CCATG 5297095 1.953484 7.247202 55-59
CCGCC 4706975 1.9441378 6.3733068 3
GAACA 7599880 1.9309467 5.4461093 60-64
ATTTT 3633955 1.9217746 5.9659195 1
GGTGG 8380495 1.9093174 7.5660057 2
TTTAA 4443940 1.903807 5.634913 3
CATTT 3768110 1.8980992 5.426025 105-109
GGTTC 5128910 1.8840708 6.065893 50-54
GGCCG 6968240 1.8740109 5.8497677 35-39
ATATT 4361645 1.8685517 7.382778 2
CCTCC 3458700 1.8586332 6.22994 120-124
AAATA 6588845 1.8523707 5.1132784 10-14
TTTGT 3645535 1.8291804 5.8682346 3
GCCTC 4207440 1.8244443 5.1390595 120-124
AGCAG 7494635 1.8067019 6.701915 2
TTCAA 4331130 1.767374 7.887279 5
CTCTG 3825450 1.741497 5.220879 120-124
CCTCT 3084830 1.7403618 5.5889387 80-84
TGAAG 6842140 1.7316315 5.319573 30-34
TCAAA 5176265 1.7111012 5.7576914 6
ATTCA 4141865 1.6901419 7.840311 4
TCTCC 2936265 1.6565462 5.513213 8
AGCCT 4430010 1.6337172 7.4850864 1
TGGGT 5489920 1.6273091 5.2504463 9
CCAAG 5402765 1.6140617 5.3081985 1
CAGCA 5401560 1.6137017 7.7365174 1
CATGT 4159545 1.6104436 5.923725 55-59
CAAAT 4849640 1.6031296 5.847685 10-14
TTGTT 3193810 1.6025233 6.4199066 4
ATTCT 3146945 1.5852015 8.188396 4
GCCCT 3610690 1.5656796 7.2565 140-142
TGTTT 3119010 1.5649917 5.6147556 120-124
GAAAG 7530710 1.5439433 6.4131284 140-142
GCAGG 6681045 1.5281037 6.1353974 3
GCTGG 5319075 1.5018017 5.0517364 2
TGGTA 4787480 1.4956795 14.311737 1
CAAAC 4742365 1.4932297 6.182637 7
AATAT 4256750 1.4772869 5.804504 1
TCAGT 3739955 1.4479916 6.7127004 80-84
CTTTA 2851605 1.4364307 6.412523 2
ACTCT 2961310 1.420861 6.6326284 120-124
GCAAA 5540055 1.4075948 11.567525 9
TTGCC 3081260 1.4027121 6.234109 125-129
TTTCA 2770965 1.3958101 5.9024777 130-134
TTCTC 2356135 1.39552 8.991183 5
AACGA 5468730 1.3894727 5.396487 20-24
TAGTA 4178860 1.3759955 14.849803 4
CGCCC 3320420 1.3714442 5.1468434 120-124
GCAGT 4572360 1.3606439 5.1524982 65-69
TTAAT 3165275 1.3560206 5.406612 135-139
ATGCC 3656925 1.3486158 6.560928 140-142
CCTTG 2945405 1.3408656 5.6986127 4
GTCAG 4504210 1.3403637 5.2543063 80-84
CGAAA 5240835 1.3315701 7.3868737 140-142
GTAGC 4447525 1.3234955 13.309832 6
ACCCC 3031125 1.3195217 5.2052817 115-119
TCATT 2618870 1.3191959 6.3536744 125-129
ACGAG 5197635 1.2529731 5.056605 20-24
CCCTT 2191350 1.2362891 7.086468 3
GTGGT 4145650 1.2288438 9.19089 3
ATCAA 3644245 1.2046663 5.1135178 130-134
CTATT 2381280 1.1995153 8.088854 2
AGTAG 4738765 1.1993024 11.751245 5
CTCAT 2482290 1.1910235 7.0442185 135-139
TTCAT 2354165 1.1858568 5.4748116 125-129
CAGAC 3968105 1.1854608 5.1011024 3
TCCGC 2721000 1.1798893 5.511888 2
GTAGT 3767175 1.1769211 14.068895 3
CGCTC 2709520 1.1749113 5.894791 135-139
AGCAA 4556955 1.1578127 11.503963 8
GCTCA 3102820 1.1442705 5.6028423 135-139
CCTAG 3082790 1.1368839 6.6338344 3
GCCTA 3064215 1.1300336 7.032519 2
TCTCA 2345735 1.1255033 7.5809517 6
CGATG 3746610 1.1149172 5.7161565 125-129
CGGTC 3141245 1.0991234 5.348396 85-89
AGGGC 4781925 1.0937327 6.098855 9
TAGCA 3480770 1.091709 13.908138 7
CCGAC 2932595 1.030141 5.6739717 130-134
TATAG 2978240 0.9806609 5.596316 140-142
CCGAT 2651055 0.9776668 7.5822525 135-139
TGCCG 2764085 0.96715486 5.302031 140-142
GGTAG 3877220 0.9310145 10.807143 2
TGCCC 2127660 0.92260313 7.405896 140-142
TCGGT 2501175 0.9187899 5.9751577 85-89
TCCGT 1932645 0.87981695 6.8229637 135-139
CTCAA 2253675 0.87597364 6.183111 7
CCTAT 1815825 0.871248 7.774365 1
CATTA 2110365 0.8611619 5.0697007 125-129
TCAAG 2569910 0.8060268 5.1188164 140-142
CTAGG 2570875 0.76504153 6.6043215 4
ATCGA 1478590 0.46374512 6.125247 140-142
GATCG 1450650 0.43168473 5.9065614 140-142