FastQCFastQC Report
Mon 16 Oct 2017
E743-IP-2-mRNA_all.fq.clean_fq_reverse

Summary

[OK] Basic Statistics

Measure Value
Filename E743-IP-2-mRNA_all.fq.clean_fq_reverse
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 27878730
Sequence length 16-147
%GC 50

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAG 329604 1.182277671902558 No Hit
TGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAG 202344 0.7258006372600186 No Hit
CCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAG 121120 0.43445307587540755 No Hit
AATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTG 119020 0.42692045154137226 No Hit
AGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCTTGGTG 114447 0.4105172653130182 No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGA 86798 0.311341298545522 No Hit
CAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACA 86661 0.3108498844818254 No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGGGGAGG 86123 0.3089200978667249 No Hit
ACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGA 84523 0.30318095551698376 No Hit
GGCCCTTGAAAATCCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTAC 81931 0.293883544910403 No Hit
CCTTGAAAATCCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTACCCA 80706 0.28948951404888246 No Hit
TGAAGCCTAGGGCGCGGGCCCGGGTGGAGCCGCCGCAGGTGCAGATCTTG 78764 0.2825236300218841 No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGG 75948 0.2724227394863396 No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG 71565 0.25670107641201734 No Hit
AGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTA 71045 0.25483585514835144 No Hit
GGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTA 56582 0.20295759527065976 No Hit
TTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTC 55782 0.20008802409578916 No Hit
TGGCGTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGT 55459 0.19892943473393518 No Hit
TGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTG 52612 0.18871734831536444 No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA 50841 0.18236483512699467 No Hit
TAGTGACGCGCATGAATGGATGAACGAGATTCCCACTGTCCCTACCTACT 49739 0.17841200083361042 No Hit
GCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGG 43351 0.15549847500226877 No Hit
TGGCCCTTGAAAATCCGGGGGAGAGGGTGTAAATCTCGCGCCGGGCCGTA 41011 0.14710497931577227 No Hit
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTG 39982 0.14341399339209498 No Hit
GCATTGAATGAATTTAAATCATCTTCTTTGGCAGTAATCCATCTTTGACT 39210 0.14064485720834485 No Hit
TGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCG 38256 0.13722289358231168 No Hit
TGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGA 35871 0.12866798451722872 No Hit
GTGGCATTGAATGAATTTAAATCATCTTCTTTGGCAGTAATCCATCTTTG 35852 0.12859983220182555 No Hit
ACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGT 34777 0.1247438459355932 No Hit
TATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAA 34466 0.12362830014136227 No Hit
GGAGCCCCGCGGACGCTACGCCGCGACGAGTAGGAGGGCCGCTGCGGTGA 34281 0.12296471180717342 No Hit
AGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGCGCTCTCGCAGAC 34046 0.12212177527455517 No Hit
CCGCGACGAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCG 33889 0.12155862193148684 No Hit
GTGGAGCCGGGCGTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGC 33702 0.12088785966936084 No Hit
AATTAGTGACGCGCATGAATGGATGAACGAGATTCCCACTGTCCCTACCT 32789 0.11761296156603979 No Hit
AGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGA 31718 0.11377132315568177 No Hit
AGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTCGGC 31005 0.11121381784607837 No Hit
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATT 30250 0.10850566004979424 No Hit
GATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAG 29163 0.10460663021593883 No Hit
CGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCT 29130 0.1044882604049754 No Hit
TTGGCAGTAATCCATCTTTGACTTAAGGCAATACAAGCATTTGTCAGAGT 28812 0.10334760586296435 No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCA 28572 0.10248673451050316 No Hit
CCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGAAGTC 28411 0.10190923331156046 No Hit
GATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG 28052 0.10062151324683728 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
ACTTT 5685615 3.1037042 8.035385 25-29
CTTTG 5697360 2.9940479 8.3173685 25-29
CGCCG 7339550 2.8157032 6.176498 4
TGAAC 8269285 2.8079114 10.260107 70-74
TTTTT 3951280 2.7626958 8.846275 130-134
TTCCC 4032445 2.583397 5.531277 7
TTGAA 7338085 2.5654037 7.725887 30-34
TTTTC 3557665 2.4160326 8.478396 130-134
AACTT 5473460 2.4017622 9.1012335 25-29
TTGGT 5684955 2.3802195 5.8247848 45-49
GTTTT 4281900 2.3167498 6.638404 105-109
TTTTG 4199790 2.2723236 5.508881 2
GTTCC 4436485 2.26447 6.551653 100-104
CTGGC 5711455 2.2559097 6.046096 85-89
GAACT 6583810 2.2355928 7.128704 25-29
TATTC 3910110 2.134479 14.9417305 3
TCCCT 3303725 2.1165404 5.9646616 8
CCCTC 3377720 2.101787 10.345383 140-142
GAACA 7505840 2.0487072 6.0309925 60-64
CCATG 4934390 2.0245392 8.589105 55-59
TGTTG 4814300 2.0156872 6.7344594 5
GGCGC 6457310 1.9736673 5.527141 90-94
TGGTT 4711015 1.9724429 5.0626435 130-134
TGGTG 6075605 1.9684612 12.333579 1
CCTCC 3151005 1.9607136 6.6785803 120-124
ATATT 4306220 1.9454578 6.6642246 2
GGTGG 7748195 1.9426084 9.798475 2
TTTGA 4454070 1.937157 5.695479 25-29
GGTTC 4758275 1.9350046 7.082924 50-54
GCCGC 5031930 1.930421 5.3096848 25-29
GGCCG 6252220 1.910982 6.577465 35-39
ATTTT 3360615 1.8887705 5.3310833 1
GCCTC 3809010 1.8883486 5.3328114 120-124
AGCAG 7104050 1.8666755 7.4595475 2
CCGCC 3875780 1.8662578 6.6504645 3
CCTCT 2870130 1.8387566 6.127638 80-84
TTTGT 3374790 1.825952 5.3535542 3
AAATA 6226780 1.8176885 6.0088263 10-14
TTCAA 4137560 1.8155675 8.545052 15-19
TCCTG 3489630 1.7811764 5.264717 85-89
TCTCC 2779925 1.7809666 7.1572022 8
TGAAG 6537235 1.7685379 6.2333045 30-34
GGCCC 4593175 1.7620995 5.4321156 1
ATTCA 3982835 1.7476737 8.421062 15-19
TCAAA 4916075 1.7340108 5.2716255 6
CATGT 4088195 1.7269583 6.9914384 55-59
TCCAT 3223185 1.7089548 5.4270325 50-54
CAGCA 5181365 1.7088428 8.916463 1
AGCCT 4120270 1.6905127 6.920407 1
CTCCA 3238130 1.6675625 6.1374664 9
TTCCA 3106370 1.6470188 5.499193 50-54
CAGCC 4130595 1.6460683 5.3433013 8
CCAAG 4911955 1.6199896 5.8302655 1
CAAAT 4557675 1.607595 6.981224 10-14
TTGTT 2946865 1.5944204 5.861948 4
GCCCT 3185070 1.579025 8.359494 140-142
GCCGA 4950855 1.5718834 5.243743 35-39
CAAAC 4567915 1.5649259 5.8018603 7
TGTTT 2865375 1.5503298 5.144092 120-124
GCAGG 6125770 1.5495504 6.896995 3
GGGTT 4760195 1.5422759 5.335369 50-54
CGGCC 4011715 1.5390315 5.002261 135-139
ATTCT 2810195 1.5340493 8.226926 4
TGGTA 4529485 1.5244175 17.294352 1
AATAT 4153000 1.5081784 5.1890736 1
TCAGT 3537175 1.4941933 8.16557 80-84
GAAAG 6844430 1.4884108 5.958818 140-142
GCAAA 5295125 1.445296 13.9557085 9
GTCAG 4403145 1.4393308 6.3574047 80-84
TTCTC 2178890 1.4371943 9.255069 5
TAGTA 4102740 1.4343227 17.771713 4
CTTTA 2617830 1.4290398 5.5083284 2
GCAGT 4309910 1.4088535 6.0364356 65-69
ATGGG 5402770 1.4070807 5.1204095 75-79
AATCC 3274480 1.395574 5.0392594 8
TTGCC 2725520 1.3911595 6.5335717 125-129
ACTCT 2595785 1.3763031 6.0247636 120-124
AACGA 5039875 1.375626 6.306294 20-24
ACCCC 2707530 1.3542647 5.8342433 115-119
GTAGC 4137555 1.3525128 16.429533 6
CATGG 4114125 1.3448538 5.783602 75-79
CCTTG 2621720 1.3381778 6.121865 4
ATGCC 3260605 1.3377992 7.496372 140-142
TTTCA 2445195 1.3348006 5.499264 130-134
CAGTC 3247965 1.3326131 5.0457573 80-84
TCAGA 3920045 1.3310871 5.577161 5
TTAAT 2923885 1.3209486 5.208549 135-139
TCATT 2384330 1.3015752 5.9709587 125-129
CGCCC 2701700 1.3009173 5.090059 120-124
GTGGT 3940625 1.2767397 12.089501 3
ACGAG 4819485 1.2663783 5.9537807 20-24
GTCTC 2451610 1.2513503 5.5302815 7
CCCTT 1946110 1.2467806 7.748418 3
CGAAA 4563405 1.2455742 6.9441347 140-142
AGTAG 4535690 1.2270539 14.069886 5
GTAGT 3632195 1.222431 17.067541 3
CTCAT 2282980 1.2104517 6.931454 135-139
AGCAA 4412175 1.2042962 13.890688 8
TCCGC 2420670 1.2000673 5.740862 2
CTATT 2182975 1.1916581 8.169131 2
CGCTC 2378125 1.1789753 6.0248685 135-139
CAGAC 3540700 1.1677424 5.647619 3
TAGCA 3409660 1.1577812 17.009407 7
TTCAT 2118385 1.1563993 5.1781535 125-129
CCTAG 2812930 1.1541219 6.1034155 3
GCTCA 2809415 1.1526798 5.5675464 135-139
CAGAT 3368475 1.1437966 5.158718 1
CCCCA 2286235 1.1435394 5.138334 115-119
GCCTA 2771850 1.1372672 6.451765 2
CGGTC 2850300 1.1258111 6.610227 85-89
TCTCA 2117930 1.122941 7.802507 6
CGATG 3361915 1.0989662 5.8277855 125-129
AGGGC 4298635 1.0873655 5.9215317 9
CGTGG 3285760 1.0339884 5.4443226 7
CCGAC 2532220 1.0091056 5.61146 130-134
TGCCG 2515285 0.9934869 6.166097 140-142
GGTAG 3743990 0.9750733 13.220025 2
CTCCG 1959910 0.9716417 5.1927595 120-124
CCGAT 2367710 0.9714517 8.299319 135-139
TATAG 2768760 0.96796197 6.0414186 140-142
TCGGT 2376120 0.96627516 7.39422 85-89
TGCCC 1911140 0.9474636 8.5085745 140-142
TCCGT 1754805 0.89568734 7.3568854 135-139
CCTAT 1678870 0.8901484 8.030826 1
CTCAA 2061320 0.8785288 6.2773266 7
GATGC 2644970 0.86460626 5.375063 140-142
TCAAG 2332855 0.79214233 5.161051 140-142
CCTCG 1586130 0.7863371 5.3677993 130-134
CTAGG 2371910 0.7753465 6.216108 4
TCGAA 1694705 0.57545257 5.0879 140-142
ATCGA 1355025 0.46011114 6.3561215 140-142
GATCG 1255300 0.4103412 6.2814384 140-142