LOCUS AeDCL1 8494 bp DNA linear PLN 24-DEC-2022 DEFINITION Complete genomic sequence of DCL1 for Cultivated Okra. ACCESSION AeDCL1 VERSION KEYWORDS . SOURCE Abelmoschus esculentus ORGANISM Abelmoschus esculentus Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Malvoideae; Abelmoschus. REFERENCE 1 (bases 1 to 8494) AUTHORS Magdy,M. TITLE Direct Submission JOURNAL Submitted (24-DEC-2022) Genetics, Faculty of Agriculture, Ain Shams University, Hadayek Shubra, No. 68, Cairo, Cairo 11241, Egypt COMMENT Bankit Comment: ALT EMAIL:m.elmosallamy@gmail.com Bankit Comment: TOTAL # OF SEQS:4 ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..8494 /organism="Abelmoschus esculentus" /mol_type="genomic DNA" /cultivar="OEL12" /db_xref="taxon:455045" /country="Egypt" gene 1..8494 /gene="DCL1" CDS join(1..1071,1169..1282,1784..1996,2118..2513,2606..2836, 3076..3249,3342..3464,3562..3729,3777..4034,4121..4285, 4357..4536,4694..4858,4941..5033,5140..5811,6020..6193, 6281..6475,6556..7470,7595..7765,7863..8234,8318..8494) /gene="DCL1" /codon_start=1 /product="DICER-Like Protein" /translation="MEGEGRVSGGNGSSYWLDACEDIPCDLINDFIDFDAPLVQDSVD NTSGQDFFGGIDHILDSIKNGGGLPPVEGNGNSSVVNGNGIHDTVVGDGFCSNELTGV SKSLPENLVPPPNGVEKSSIESKGQEKKCDVTNSNLFDYSTKDNRVRRDYKRSSESRD RGLDSKERCDKRSSTNGCKNDWQYSSRGQYHPRDRERYSARKRVRDWDDIDRRGREHV RRREHYYGGNRRDGRDREPRGYWERDRSGSNEMVFRLGTWEADQQREAKAANDKTQES NGKMEKKVEQPKEMVLEEQARQYQLDVLEQAKRKNTIAFLETGAGKTLIAVLLIKSIA DDLQKQNRKVLSVFLVPKVPLVYQQAEVIREQTGFQVGHYCGEMGQDFWDARRWQREF ETKQVLVMTAQILLNILRHSIIKMETIHLLILDECHHAVKKHPYSLVMSEFYHTTPKE KRPSVFGMTASPVNLKGVSSQVDCAIKIRNLESKLDSVVCTIKDRKELEKHVPMPSEV VVEFDKAASLWSLHEQIKQMELTVEEAAQSSSRRSKWQFMGARDAGAKEELHQVYGVS ERTESDGAANLIQKLRAINYALDELGQWCAYKVAQSFLTALQNDERANYQLDVKFQES YLNKVVSLLQCQLSEGAVTVKDMSTSEAERCNAQDGTIPDEIEEGELPDSHVVSGGEH VDVIIGAAVADGKVTPKVQSLIKILLKYQHTEDFRAIIFVERVVAALVLPKVFAELPS LGFIRCASLIGHNNSQEMRTGQMQDTIAKFRDGRVTLLVATSVAEEGLDIRQCNVVIR FDLAKTVLAYIQSRGRARKPGSDYILMIERYLKTNFHIVYRGNLSHATFLKNARNSEE TLRKEAIERTDLSHLKDTSRLISMDKIQGTVYQVESTGAIVSLNSAVGLVHFYCSQLP SDRYSILRPEFIMKKHEKPGGPIEYSCKLQLPCNAPFEELEGPMCSSMRLAQQACCLA VCLAACKKLHEMGAFTDMPLPDKGSGEEAEKADQNDEGDPLPGTARHREFYPEGVADI LQGEWILSGRGGTGDTKLHRLYMYTIKCVNNGSSKDPFLTKVSDFAVLFGKELDAEVL SMSVDLFIVRAMITKASLVFRGSIDITGSQLASLKCFHVRLMSIVLDVDVDPATTPWD PAKAYLFVPVVGDKCVDPIKEVDWDLVDNIITTDAWNNPLQRARPDVFLGTNERTLGG DRREYGFGKLRHGLAFGHKSHPTYGIRGAIASYDVVKATGVVPSRDAIEVQEGDLTKG KLIMADGFAQADILVGRIVTAAHSGKRFYVDSICYDMTAETSFPRKEGFLGPLEYSSY ADYYKQKYESAFFLTYIFPSFMKYAFCFFPHNGECIRLLSPLHIVTRYGVELRYKRQA LMRGRGVSYCKNLLSPRFEHSEGESEEAFDKTYYVFLPPELCFVHPLPGTLVRGAQRL PSIMRRVESMLLAIQLKHIIQFPVPAAKVLEASTSASCQETFCYERAELLGDAYLKWV VSRFLFLKYPDKHEGQLTRMRQQMVSNMVLYQYALNKGLQSYIQADRFAPSRWAAPGV LPVFDEDTKDGDTSLFDQEHATVDVLPGKVHGDGFEDEDMEDGEIESDSSSYRVLSSK TLADVVEALIGVYYVEGGKSAANHLMKWIGIQVESDPVVMESIVKPSNIEENLLKSVN FDALEGALNIKFKNRALLVEAITHASRPASGVSCYQRLEFVGDAVLDHLITKHLFFTY TNLPPGRLTDLRAAAVNNENFARVAVKHQLHVHLRHGSSALEKQIRDFVKEVQNELLK PGFNSFGLGDCKAPKVLGDIVESIAGAIFIDSGRDTSVVWRVFQPLLHPMVTPETLPM HPVRELQERCQQQAEGLEYKASRSGNLATVEVFVDGVQVGVAQNPQKKMAQKLAARNA LAVLKEKETTEAKETIEENGKKMKNGNQTFTRQTLNDICLRRNWPMPSYRCVSEGGPA HAKRFTFAVKVNTADRGWTDECIGEPMPSVKKAKDSAAVLLLELLNKWYS" BASE COUNT 2359 a 1462 c 1963 g 2710 t ORIGIN 1 atggagggtg agggtagggt ttctggtggc aatgggtcat cttactggct ggatgcttgt 61 gaggacatac cgtgcgactt gattaacgat ttcattgatt ttgatgcccc tctagtccaa 121 gactcagttg acaatacttc aggtcaagat ttctttggtg gaattgatca cattctcgac 181 agcatcaaga acggcggtgg ccttcctcct gtggaaggta acggtaatag ctctgttgtc 241 aatggaaatg gaattcatga cactgttgtc ggggatggat tttgttccaa tgagctcact 301 ggggtttcca agagtttacc tgaaaacttg gttccaccgc ctaatggtgt tgaaaagagc 361 agcattgaaa gcaaaggaca ggagaagaaa tgtgatgtta ctaattccaa tttgtttgat 421 tattccacta aggataacag agttcgtcga gactataaaa ggtcatctga gtcaagagat 481 aggggtttag atagtaaaga gaggtgtgac aagaggtcta gcaccaatgg ctgcaagaat 541 gattggcagt attctagtag agggcagtat catcccaggg atagggagag atattctgct 601 agaaaaaggg ttcgtgattg ggatgatatt gataggaggg gtagagagca tgttagaaga 661 agagaacatt attatggtgg taataggagg gatgggagag atagagaacc aaggggttat 721 tgggagaggg atcggtcagg ttccaatgaa atggttttta ggttgggaac ttgggaagca 781 gatcagcaaa gagaagcgaa ggcggctaat gacaaaacac aggagtccaa tggaaagatg 841 gagaagaaag ttgaacagcc aaaggaaatg gttttggaag agcaagctcg tcaatatcaa 901 ttggatgttc ttgaacaggc aaaaaggaaa aacacaattg cttttcttga aactggggca 961 gggaagacac tcattgctgt tctcctcatc aagagtattg ctgatgattt acaaaagcag 1021 aacagaaaag tgctctctgt ctttttggtt cctaaagttc cactagttta tcaggtacta 1081 gaatttgtag aattttaatg ttcctttaag atttgactgg cttttttgtt ctcaggttca 1141 gttcttctta ttgtttattt acattttaca gcaagctgaa gttattcgcg agcaaactgg 1201 tttccaagtc ggccattatt gtggggagat gggtcaagac ttctgggatg ctcgtaggtg 1261 gcagcgcgag tttgaaacca aacaggtaat atctcaaatc ctttttgtga attcctcctt 1321 gactgccatt tgagcttttc ttcaatgttc caagtaattt cctcattgta atgctagtcg 1381 ccacttaagc aaactgcatt atgttacttg gtaattttta aatatgccat aaggagcatt 1441 acgaaaatgt tatatatctg atcggcggta acacttctac tatgactagc tttacacttt 1501 agttttgtcg aacgtttgat tcggatttta ctcaattttg ttgaaggaat gggatgctgt 1561 tgatatagtt ttcaaatgct agcaaggtcc ttgtatgtct actttataga gttggaattt 1621 agttgaaata taagtggtat acccttcatc ccattttaaa atgaaaaggc gaactctata 1681 tcttcaaaat gattttatct tttcatgttt ttgtgagctg ttgattttgc atacctgact 1741 ttatctatat gtctatataa tttgctgaat tgatgttctc ttgcaggtct tggttatgac 1801 agctcaaatt cttctgaata ttttgaggca cagcataatt aaaatggaaa caatccatct 1861 tcttattctg gatgagtgtc atcatgcagt gaagaaacat ccgtattcac tggttatgtc 1921 tgaattctat catacaacac caaaggagaa gagaccttct gtttttggaa tgactgcttc 1981 tcctgttaac ttgaagggta tatttttatt ttcaagtaat gactttatcg aatttatatt 2041 tcataaagac ttgcaaaatc ttctttcagt tgattgtaac aaaaattccc ctgcaaatat 2101 atttttgaat atattcaggt gtttcaagtc aagtcgattg tgcaataaag attcgtaatc 2161 tagaaagcaa gctggattcc gtagtctgta ctattaaaga ccgcaaggaa cttgagaaac 2221 atgtgccaat gccctcagaa gttgtggtag agtttgacaa ggcagccagt ttatggtccc 2281 ttcatgaaca aataaaacaa atggaattga cagttgaaga agctgcacag tcaagttcta 2341 gaagaagtaa atggcagttc atgggagcta gagatgcagg agccaaggaa gagcttcacc 2401 aagtttatgg tgtatctgaa agaacagaaa gtgatggagc tgctaatttg atacaaaagt 2461 tgagggccat taattatgca cttgatgaat tgggtcagtg gtgtgcttac aaggtattgt 2521 atgccttaaa tgattaagtt tccattcttt tattttcttg attgtgtaaa aactatcttg 2581 gttcctttta tttgcctttt tgcaggttgc acaatctttt ctgacagctt tacaaaatga 2641 tgaaagggca aactaccagc ttgatgtcaa gtttcaagaa tcttacctaa acaaagttgt 2701 ttctctctta caatgtcaat tgtcggaggg agctgttacc gtaaaagata tgagcacttc 2761 agaagcagag aggtgtaatg ctcaagatgg gaccatccct gatgagattg aggaaggaga 2821 actccctgac agtcatggtg catcttttgt ttaattatct acctttcttt tgttaattta 2881 ctaccacaaa ttgaggctga ggccatttgt tcctgatttt ggaaaactga tttccacatg 2941 cactgctgtc ttgctcgtgc tgtgtttctt gtcttctttt ataacgaaat cttttatcat 3001 ctaagatatt tctaacagaa aataatttaa gagcattttg gtgtgtattc tcaactcgtt 3061 ttcctttttg tcatagttgt ctctggtgga gagcatgtgg atgtgataat tggggcggct 3121 gtagcagatg gtaaagtgac cccaaaagta cagtcattga ttaaaatact tctcaagtat 3181 cagcacacgg aggattttcg tgcgatcatc tttgttgagc gagttgtggc tgctttagtt 3241 cttcctaagg ttgaaggatt gacatgtttt attatgttca atgatatatt catccccttg 3301 cactttcatg gtaatgctat taattctctt tgaatggaca ggtttttgca gagcttccat 3361 ctctgggttt catcagatgt gctagtttga ttgggcacaa caatagtcaa gaaatgcgga 3421 ctggacaaat gcaggataca attgctaaat tccgagatgg tcgtgtatgt taatattcca 3481 tgcttcctct ctacagctga taaaatatat gaatcatgca attgtagaca tttgctaaaa 3541 tgtttttgtt ttctgttgta ggtgacattg ttagttgcga ctagtgttgc ggaggaagga 3601 ttggatattc ggcaatgcaa tgttgttatt cgttttgatc ttgcgaaaac agttttggca 3661 tacattcagt ctaggggtcg tgcaaggaag cctgggtcag attacatctt aatgattgag 3721 aggtacttgt tgggtttggt ctttaagcat tttctttcta ccataaatga tccgtaaaaa 3781 ctaactttca tattgtctac agaggaaatt tatcgcatgc tacattccta aagaatgcaa 3841 ggaatagtga agagaccttg cggaaagaag caattgagag aactgatctt agtcatctga 3901 aggatacttc gaggttgatt tcgatggata agatacaagg tacagtgtac caggttgaat 3961 caactggtgc tattgtcagt ttaaattctg ctgttggtct cgtccacttt tattgctctc 4021 agcttcccag tgacaggtat tgcacttata taagatgaca tttgttgttg tttctcttgt 4081 agtttgttct tttacttagg tgccattgat tttggctttt agatattcaa tactccgacc 4141 tgagtttatt atgaagaagc atgagaagcc agggggtcca attgaatatt cttgcaagct 4201 tcagctccct tgcaatgcac catttgaaga gcttgagggt cccatgtgca gttctatgcg 4261 ccttgcccag caggcatgct gtcttgtcac tatgtacttc tctataaagc atgtaataat 4321 agtattcctt atgttggtaa tatgtgtaac atgtaggctg tgtgtttggc tgcttgcaaa 4381 aagctccacg agatgggagc gtttactgac atgcccttgc ctgacaaagg aagtggagaa 4441 gaagcagaga aggctgacca gaatgatgaa ggagatccac ttcccggaac tgctagacat 4501 agagagttct atcctgaagg tgtagcagat atactccagg ttagtgtagt ccatggattt 4561 gacttcccat ccatgtactt ttaagggatt taccttttat tgtgcttatg gagggagatg 4621 tcggaggaaa ccaacatggc ctttaactaa tgtgcatttt ggtttgcaag tttattgaat 4681 gctttctggt cctcagggtg aatggatttt atctggaaga ggtggcactg gtgacacgaa 4741 actacatcgt ctctacatgt ataccatcaa atgtgtaaat aatggctctt caaaagatcc 4801 attcttaact aaagtctcag attttgcagt actttttggc aaagagctgg atgcagaggt 4861 aatttcattt gttattatat tagcataaca tgcatttggt aaataagata ttaattttga 4921 ataacatcca tctcaattag gtgttatcga tgtcggtgga tctatttatc gttcgagcca 4981 tgataaccaa ggcatctctt gtcttcaggg gatcaataga tattactgga agtcaggtga 5041 gatgtctaaa ttgtttggat attgattaca attagacaat tttttggtgt ttctatttca 5101 gattatactt gtgttataat tctgaagtct gcttatgtgc agttggcatc ccttaaatgt 5161 tttcacgtaa gactgatgag cattgtactg gatgtggatg ttgatcctgc cactactcct 5221 tgggatcctg ccaaggcata tttgtttgtc cctgtggttg gcgataagtg tgtagatcct 5281 ataaaagaag ttgattggga tttggttgat aatataatca ctacagatgc atggaataat 5341 ccccttcaga gagctaggcc agatgttttc cttggaacaa atgagaggac gcttggtggt 5401 gacagaaggg agtatgggtt cgggaaattg cgtcatggtc tggcttttgg gcacaaatct 5461 catcctacat atggtatcag aggagctata gcctcatacg atgttgttaa agctactggg 5521 gtggttccta gtcgtgacgc aattgaggta caagaagggg atttgaccaa aggcaaattg 5581 ataatggctg atggttttgc acaagcagat attcttgttg gaagaatagt aacagctgct 5641 cattcaggga agaggtttta tgtagattca atatgctatg acatgacagc agagacctcc 5701 tttccgagga aagagggctt ccttggtcct ctggagtaca gctcatatgc tgattactat 5761 aagcagaagt acgaatctgc attcttcctt acctatatat ttccttcttt cctcccttga 5821 tattaaaatt agaagtaaca attggctgcc tattaattgc ttctgtttaa tggatgagat 5881 ggcctacatg cacctcagtt gtgcgtgaag aaaaggaaag catgtcttac actgcgaggg 5941 acatggttga ctactattgg gtgcagaata tgtgagtttt agtcacattt ctacctaata 6001 gtagtaaatg tcctcacaaa tgaaatatgc tttctgtttc tttccgcaca acggagagtg 6061 cattaggcta ttgagtcctc tccatattgt taccaggtat ggagttgagt tgagatacaa 6121 acggcaagct ttaatgagag gtcgtggtgt ttcatattgc aagaatctct tatcccctcg 6181 attcgaacac tcagagggta catttaacaa aattcttctt gcgtctgaat gttataaatt 6241 tctatgatcc ttttgttgct taacgtaatg tgtaccatat gaaggtgaat cagaggaggc 6301 ttttgacaaa acatactatg tttttcttcc tcctgagctg tgttttgtcc acccgcttcc 6361 tggaacactt gttagaggtg cccagaggtt gccctctatt atgaggaggg tcgagagcat 6421 gcttcttgca attcaactta agcacataat acaatttcca gtccctgctg caaaggtgcg 6481 atatattctt tggttggtca aatccgagca gctttcagat tattgataaa cttttaattt 6541 cttttgtatg ttcaggtttt ggaagcttcg acttccgctt catgtcagga gacattttgc 6601 tatgaaaggg cggagcttct gggggatgct tacttgaaat gggttgttag tcgctttttg 6661 tttcttaaat atcctgataa acatgagggt cagctgacca ggatgagaca acaaatggtg 6721 agtaacatgg tattgtatca atatgcacta aacaagggac ttcaatcata tatccaagca 6781 gatcgctttg caccatcaag atgggctgct cctggggtat tgccagtctt tgacgaggat 6841 acaaaagatg gagacacctc cttatttgat caagaacatg caactgttga tgttttacca 6901 ggaaaagtac atggtgatgg gtttgaagat gaagatatgg aagatggtga aattgagagc 6961 gactcaagtt cttatagagt cctctctagc aagaccttag cagatgttgt tgaagcacta 7021 attggagtat attatgttga aggtggcaag agtgcagcga accacctcat gaaatggatt 7081 gggatccagg tggagtctga tcctgttgtg atggaatcta tagtgaaacc atccaatatt 7141 gaagaaaact tacttaagag tgtaaatttt gacgccttag aaggtgcatt gaacatcaag 7201 tttaaaaata gggctttgtt ggtagaagct attactcatg cttcacgccc agcatccgga 7261 gtatcctgct accagcgctt ggagtttgtt ggtgatgcag tcttggatca tcttatcaca 7321 aaacatttat ttttcacata cactaatcta cctccaggtc gcttgactga tttgcgagct 7381 gctgctgtaa acaatgaaaa ctttgcccgt gttgcggtaa aacatcagct ccatgtgcac 7441 cttcggcatg gatcaagtgc ccttgagaaa caggtttgtt atctgattcc ttcacttttt 7501 cttatctaat gggtggcagt tttaaccccc atcaacttgt ctgttctggt acataggtag 7561 ttttttctga ctttggtatt cttggcttct gtttcagatt cgggactttg tgaaggaagt 7621 ccagaatgag ttattaaagc cggggttcaa ctcattcggt ttgggagatt gcaaagctcc 7681 aaaagttctt ggagatattg ttgaatccat tgctggtgcc atttttattg atagcggacg 7741 tgacacttca gttgtctgga gggttagttt gggactgaaa tttgcccctt ctctactttt 7801 cttacagcta tatacaatca caataactgg ttagtaaaat agcttttctt gctttttagg 7861 tttttcaacc actgttgcat cccatggtga ctccagagac attgccaatg catcctgtcc 7921 gggaactaca agagcgatgc cagcaacaag ctgaaggctt ggaatacaaa gcaagtcgaa 7981 gtggcaattt ggccacagtg gaggttttcg ttgatggtgt ccaagtggga gttgctcaga 8041 atccccaaaa gaagatggca cagaaactcg ctgcgaggaa tgcgcttgct gttttgaagg 8101 agaaggaaac aactgaagct aaggagacca tcgaagagaa cgggaaaaag atgaagaatg 8161 gcaaccagac atttacaaga caaacactga atgatatctg cctgcgtcgt aactggccca 8221 tgccctccta tcggtaagta attagagtcg gtaccttttc ttcctcacgt acttggacaa 8281 caacagagac aacttatctt atgtgtgtat ctttaggtgt gtgagtgaag gggggccggc 8341 ccatgcgaag agatttacat ttgctgtcaa ggttaatacc gctgacagag gatggaccga 8401 tgaatgcatt ggcgagccga tgcctagtgt taagaaggct aaggactcgg ctgcagtgct 8461 tctcttggaa ctactaaaca aatggtattc gtga // LOCUS AeDCL2 5214 bp DNA linear PLN 24-DEC-2022 DEFINITION Complete genomic sequence of DCL2 for Cultivated Okra. ACCESSION AeDCL2 VERSION KEYWORDS . SOURCE Abelmoschus esculentus ORGANISM Abelmoschus esculentus Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Malvoideae; Abelmoschus. REFERENCE 1 (bases 1 to 5214) AUTHORS Magdy,M. TITLE Direct Submission JOURNAL Submitted (24-DEC-2022) Genetics, Faculty of Agriculture, Ain Shams University, Hadayek Shubra, No. 68, Cairo, Cairo 11241, Egypt COMMENT Bankit Comment: ALT EMAIL:m.elmosallamy@gmail.com Bankit Comment: TOTAL # OF SEQS:4 ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5214 /organism="Abelmoschus esculentus" /mol_type="genomic DNA" /cultivar="OEL12" /db_xref="taxon:455045" /country="Egypt" gene 1..5214 /gene="DCL2" CDS join(1..69,134..313,421..534,673..807,859..942,1030..1092, 1202..1345,1436..1564,1576..1623,1688..1780,1790..1987, 2070..2228,2365..2586,2704..2928,3055..3327,3382..3639, 3945..4547,4588..5214) /gene="DCL2" /codon_start=1 /product="DICER-Like Protein" /translation="MEPVDIEMAISRQHSFYSVLLSLSYQIEAFEKAIKQNTIAYLET GSGKTLIAIMLLRSFAYLIRKPSSFYAVFLVPQVVLVKQQADDVEMHTDLKGGNFWGD MEADFWDGAKRKQEIEKYEVLVMTPQILLNGLRHSFFKLNMIKVLVMDECHHAKRKHP YASIMTDFYHRQLEAGESNLPRIFGMTASPINTKGAKSAGGYWQKIHKLETIMNSKVY TCASESVLAKFVPFSTPKFKFYQPMKITNCLYAQLEKDLDVLKLKLLSCLLNLDLLLS AALSARNKISKRCAALKYCLEELGVWLAFKDSELLLPKCHSYNELIPDWSISNDIKAS VDAGFLTSVLCLIVSVSVCRMLKDIRCIIFVERVMTAIVLQSLFSELLSKHSNWKTKY VAGNSSGLLNQTRNKQNEIVEEFRNGMVNIIFATSILEEGLDVKSCNLIIRFDPSSNI CSFIQSRGRARMENSDYLLMVKSGDLKTQSRLKEYLTSGEVMRKESLCHASDPCSPLS KDLYDEEFYHVASTGACMTLSSSVGLVYFYCSRLPSDGYFKPSPRCVIDKQMGICTIH LPKSSPIQSVSVQGNIKNLKQKACFEACKQLHQIGALTDNLVPDIVVEEDDVEETGKE PYNDDQPIFFPPELVTQGLQESPTKYYCYLMELKQNFDYKFPVHNIMLLLKSQLEMDE KCVDVELEVDRGTLTVNVKYIGPINLDYDQVNLCRRFQIALFRVLMDRKAENLTEVLC DLTSGNSDIDYLLLPSNYVGQNPAIDWFSVSSVLFSYEKVWNYHKNCNGGMIQAKSGL YIMKCLESFHLESLETLGDSFLKYTVCQHLFKRHQNHHEGLLSVNKDKIISNTALCSL GCDKKLQGFIRDEPFDPKNWMIPGCSGIYTFNEETLCNARKIYVRGKRKVKSKRVADA VEALIGAYLSMGGEAAALLFMDWLGMTIDFTNIPYERQFKVQAEQFVNVQYLESLLNH SFQDSSLLVEALTHGSYMLPEIPGCYQRLEFLGDSVLDYLITLHFYNKYPGISPGLLT DLRSASVNNNCYALSAVKSELHKYILHGSQKLYKDIKETVQSFRDSSLEYTFGWESEK SFPKVLGDVMESLAGAIFVDSGYNKEVVFRSIRPLLEPMITPKTMTIHPVKELHELCQ KEHYELRKPVVSHENGVSSITIEVEANGRVFKQTSTASDKKMAKKLASKEVLKSLKEA NFS" BASE COUNT 1479 a 887 c 1030 g 1818 t ORIGIN 1 atggaaccag ttgacataga gatggccatt tccagacaac actcttttta ttctgtattg 61 ttgtcgctct ttgttttgtc aagacctaat gtcagaagct ttgatgttct ccgcacttag 121 ttgtttacgg agcagctacc agattgaagc atttgagaaa gctatcaagc aaaacacaat 181 agcttacttg gaaaccgggt ccggcaagac actgattgcc atcatgcttc tccgcagctt 241 cgcttacctt attcgcaagc cttcgtcttt ttatgccgtc ttcttggttc ctcaagtagt 301 cctggttaaa caagtatgta aaattacata tcctgttatt tatgtcatgc tattagtaga 361 aaattttttt aacaaagctt tttttttttt gtttctcttg ctgttttaaa taatgattag 421 caagcggatg atgtggaaat gcatacggac ttgaaagggg gcaacttttg gggagatatg 481 gaggcggact tctgggatgg tgcaaaacgg aagcaagaaa ttgagaaata tgaggtgggt 541 ttttctgttt ctaatgtata gttgatatgg aaatgatgca gagcaagtgc tttcttttcc 601 tattgtgtat ctttctttat ttcttacata tcgtacatgc agcgagcagt gacatgtctt 661 tcttaccgac aggtgcttgt gatgacacct caaattttac ttaacggatt gagacatagt 721 ttctttaagc ttaacatgat aaaggttttg gtaatggatg aatgccatca tgccaaaaga 781 aagcacccat atgcttccat aatgacagtg agttgtcttt cattcctttt tctttatatt 841 ataattttgt ttacagtgga tttctatcat cgccaattag aagctggtga atctaacctt 901 cctaggattt ttgggatgac tgcttcccct ataaacacaa aaggtgattc ttttaagctt 961 tcatgtttat tgcttgtttt tgtttctatt atatagaact cgaacatctt actcctccct 1021 attactctag gggcaaaatc ggctggtggc tattggcaga agatccataa gctggagacg 1081 attatgaatt cgattttttt tttcatttat tgcctatgct tctgatttgt ttctttgaga 1141 catgattgta tcatactgaa aattacattt taatatttgc atggtatgtt ttcctccatt 1201 gaaggtgtat acatgtgcaa gtgaatcagt gcttgctaag tttgttccat tttcgactcc 1261 aaagttcaag ttttatcaac ctatgaaaat aacaaattgc ttatatgcac aattggaaaa 1321 ggacctcgat gtattgaaat taaaggtaca tatattcttt gttgaaactt ttggctatat 1381 tgccagttta atgtgataaa tttcccctct ttttatgagg tgcgaaggag taagactttt 1441 atcatgtttg ctaaatttgg atcttctttt gtctgcagca ttgtctgcca ggaacaaaat 1501 atcaaagaga tgtgccgctt taaaatactg tttagaagag cttggtgtct ggttggcttt 1561 taaggtacat ctttagacag tgaattgttg ctaccaaagt gccattctta taatgagttg 1621 atatttagtt gctttattgt ctttttcata actgttttct ttttgttttt tatttcacca 1681 tataggtcca gattggtcca tttctaatga tattaaagcc agtgtggatg ctgggtttct 1741 tacctcagtt ttatgcctta tcgtttctgt ttctgtatgt ctacaatgca ggatgttgaa 1801 agacataagg tgtataattt ttgtagagcg ggttatgact gccattgtgc ttcagtcact 1861 attcagtgaa ttgctttcaa agcacagtaa ctggaagacc aaatatgttg caggaaatag 1921 ctctggattg ctgaaccaaa ctaggaataa acaaaatgaa attgtagaag aattccgtaa 1981 cgggatggta tgtttttatc tcatgtaaag aaggaaataa cgattttgtt acacggctgt 2041 atttgaatga tggtattcca ttcctgcagg ttaacataat ttttgctact tcaattcttg 2101 aagaaggctt ggatgtcaaa tcatgcaact taattatcag atttgatcca tcgtcaaaca 2161 tttgtagttt catacagtct cgaggacgtg ctagaatgga gaattcagat tatttgttaa 2221 tggtgaagag gtaataatct atatttctat tccttcccat atattatttc tcaaatttta 2281 aaaatcttta ttactagtgc taccttagat aacttattaa tacctttata tgttcttaac 2341 aaatgcttat ggtttaatta ttgtagcggg gatttgaaaa cacaatctcg gctgaaggag 2401 tatcttacta gtggagaagt aatgagaaag gaatcgttat gccatgcatc tgatccttgt 2461 tctcctctta gtaaggactt atacgatgaa gaattctacc atgttgcaag tacaggggca 2521 tgtatgactc ttagttctag tgttggttta gtatacttct attgctctcg tctcccttca 2581 gatgggttag taattcttag tatttactta tagtacatta taattaaagc cttattatct 2641 tattatcatt atccccatgg tttaatgttt ttgcttctct tgtgtttgtt tgttttctct 2701 aggtatttta aacctagtcc aaggtgtgtc atagacaagc aaatggggat ttgcaccatc 2761 catctaccca agagttcacc tattcaatct gtttctgtcc agggaaacat taaaaattta 2821 aagcaaaagg catgctttga agcatgcaaa cagctccatc aaattggtgc tttaacagac 2881 aatcttgtgc ctgatattgt tgtggaagaa gatgatgtag aagaaactgg taataataat 2941 gcatgcctat gttttcttta tcttcaacga tgttgtatgt gatatcaata tgcttaaaag 3001 atgttagaag aaatatctaa ctttccctca ttttatataa ttatttgatt tgtaggaaag 3061 gaaccctata atgatgatca accgattttc tttccacctg aactggttac tcagggttta 3121 caagagtctc cgacaaagta ttactgctac ttaatggagt tgaagcagaa ctttgactac 3181 aagtttccag ttcataacat catgcttctt ttgaagagtc agcttgaaat ggatgaaaaa 3241 tgtgtggatg tggaattgga agttgatagg ggtactttaa cagtcaacgt gaaatacatt 3301 ggaccaataa atcttgatta tgaccaggta ctatattctt tttttaacat ttttcctctt 3361 tgcacatatt cgtgctggta ggttaacttg tgtagaaggt ttcagattgc ccttttccga 3421 gttcttatgg accgtaaagc agaaaatttg acagaggtgt tatgtgacct tacttcaggg 3481 aattctgaca ttgattatct tcttcttcca tcaaattatg tgggccagaa tcctgctatt 3541 gattggtttt cagtttcttc tgtcttattt tcttatgaaa aggtctggaa ttatcacaag 3601 aactgtaatg gtggtatgat tcaggcaaaa agtggtttgt tatgcacttg catgattcag 3661 tcgttcagat tcttttaatt ttaaattttg ttttatttat tttgcagaat caagtaatgc 3721 attcgtagaa ttgcctcctg aactttgtga tgtccatatg actcccatat caattagcac 3781 attttattct ttcacatttg ttccatcaat catgcatagg atcgagtctt tactcattgc 3841 taccagcttg aaaaagatgc atctcgatca ttgcctgcaa aatgttgcta ttccaaccat 3901 gaaggtaaga tagcagactt tttaattcct tgcacagaaa tatgtatatc atgaagtgcc 3961 tagaaagttt tcatttggaa tcactggaga ctcttggcga ctcttttctg aagtacaccg 4021 tttgccagca tctcttcaaa agacaccaaa atcatcacga gggacttctt agtgtcaaca 4081 aggataaaat catttcgaat acagctcttt gctctctggg atgtgacaag aagcttcagg 4141 gatttatccg cgatgagcct ttcgatccca aaaattggat gattcctggt tgttcaggaa 4201 tttatacttt caatgaggag acactgtgta atgcaagaaa aatatatgtt agagggaaaa 4261 gaaaggtgaa aagtaaaagg gttgccgatg ctgttgaggc acttattggt gcatatctta 4321 gcatgggagg cgaggctgct gccttgttat ttatggattg gcttgggatg accatcgatt 4381 ttacaaatat accatacgaa agacaattca aagtgcaggc tgaacaattt gttaatgtcc 4441 agtatttgga gtcacttctg aatcattcat tccaagactc ttcattgttg gtggaagcac 4501 taacacatgg ttcttatatg cttcctgaaa ttccgggatg ttatcaggtt agtccggtga 4561 taatctagtg tattcttttc tgtgcagcgg ctggaatttc ttggggattc cgttttagat 4621 tacctcatta cactgcattt ttataataaa tatcctggga tctcgccggg attgttaacc 4681 gatttgaggt ctgcatctgt gaacaacaat tgctatgcat tatcagctgt caagtccgag 4741 ttgcataagt acattctcca tggttcacag aagctataca aggacataaa agaaactgtc 4801 caaagtttcc gggattcgtc gttggaatat acttttggtt gggaatctga aaaatctttc 4861 cccaaggtac tcggggatgt gatggagtca ctagccgggg ccatatttgt cgattcagga 4921 tacaacaagg aagtcgtatt ccggagtata aggccgctgt tggagcccat gattactcct 4981 aaaacaatga caatccaccc tgtgaaagag ttgcatgaac tatgtcaaaa ggaacattat 5041 gaactaagaa aacccgttgt ttcacacgaa aatggcgttt cttctattac gatcgaggtc 5101 gaagcgaatg gcagggtgtt caagcaaaca tcaacagcat cagataagaa gatggcaaag 5161 aaactggctt ccaaagaagt tttgaagtca cttaaagagg ccaattttag ctaa // LOCUS AeDCL3 4731 bp DNA linear PLN 24-DEC-2022 DEFINITION Complete genomic sequence of DCL3 for Cultivated Okra. ACCESSION AeDCL3 VERSION KEYWORDS . SOURCE Abelmoschus esculentus ORGANISM Abelmoschus esculentus Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Malvoideae; Abelmoschus. REFERENCE 1 (bases 1 to 4731) AUTHORS Magdy,M. TITLE Direct Submission JOURNAL Submitted (24-DEC-2022) Genetics, Faculty of Agriculture, Ain Shams University, Hadayek Shubra, No. 68, Cairo, Cairo 11241, Egypt COMMENT Bankit Comment: ALT EMAIL:m.elmosallamy@gmail.com Bankit Comment: TOTAL # OF SEQS:4 ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..4731 /organism="Abelmoschus esculentus" /mol_type="genomic DNA" /cultivar="OEL12" /db_xref="taxon:455045" /country="Egypt" gene 1..4731 /gene="DCL3" CDS 1..4731 /gene="DCL3" /note="[intronless gene]" /codon_start=1 /product="DICER-Like Protein" /translation="MHSSKEEMNSADPPDMMILDDTDAVMKEPSSSSKPKDLNPSRYQ LQVYEVAKRRNIIAVLDTGVGKTMIAVMLIKDFGETIKSADSKKLIVFLAPTVHLVNQ VFEYIKEHTSLEVEQYYGAKGVDEWTLDCWEKEVKDHDVSFLTSQILLDALRNAFLSL EKVSLLIIDECHRATGNHPYARLMKVSNSPKIFGMTASLSPPKLIVSMNNDLEYVLLL QIYTIEDRTEMEVYVPFAKESCRFYDPAELSCSDLKEMVEISWSKTDMSLSKLQDSLQ LSYKDLDDKLKALRRRISNDHAKILHCLDNLGLICAYEVDCAFLENIIDTKDECEVYR ESVVQCKNFLEEVRCLIGASLPLGDKSFLNSGFDYVKAVDLGYISPKMHELLQLFQSF GESKQVSCLIFVERIITAKVIERFTKKVSCLSHFVVSYMTGSNTSVDSLAPKMQKETL ESFRSGKVSLLFTTDVVEEGIHVPNCCYVIRFDLPKTVRSYVQSRGRARQNNSEFIMM LERGNLKQRNHIYDLIRSEYSMKNTAIKRDPGSDLCVLKDCSFEEPNIFVVDATGASV TTDSAVSLIHRYCGKLPGDKYATPKPSFEFTSSEGLYECKLTLPVNAAFQTIVGPPSR NCHLAKQLVSLEACKQLHQMGALNDHLIPSIEEPSENACVSKVKDSHSGAGTTKRKEL HGTTCIQALCGSWGEKSDDADFFAYKFDFKCSIVTVVYSQFVLLIESKLADDVGNTEM DLYLIGKTAKASVSFCGQVRLNAEQVMKAKRFQEFFFNGLFGKLFVGSKSSGTPREFL LRDKTSSLWSPSHMYLLLPLEDCSTKELRINWPAITACTFAADFLNKNSLLGTDDDGS NPSSVTDCKKTNMIHFANSLVDIDNLRNMVVLAIHTGRIYCIIEAMSDKTANSSFDEA VDKVSTGFASFSEYFRKKYVIVLKHPEQPLLLLKQSHNPHNLLVNFNDEGFSAKASQA GVVNEKPRFHVHMPPELLLVLDVPVSVLKSLYLLPSVMHRLESLMLANQLREEINFNS SNIDIPSSMILEALTTLGCCESFSMERLELLGDSVLKYTVSCTLFLKYPSKHEGQLSS RRSLAVCNSTLHKLGTVHIIQGYIRNGAFDPRRWVAPGQRTLRPVPCKCGVDSLEVPL DKIFQTEDPKVKFGKPCDRGHRWMCSKTISDCVEALIGAYYVSGGLVASLHVMKWLGV DVELDPTMVAEVINQASLQIYVPNNEIQEIESKVGHNFSVKFLLKEALTHESALEFYC YQVVEFLGDSVLDLLITQYLYHNHTDIDPGELTDLRSASVNNESFAQVVVRHDLYKHL QHCSSLLSDQISEYVQSLTESQNTSRLDPGIKGPKVMCDLFESIVGAILIDTNLNLDK VWRIVEPLLSPIVTPDKLELPPLRELNELCDSLGYFIKEKCTNKGEVVHAELLLQLDR DLLVGEGFDRSKKVAKGKAASNLLKQLEVRTRFNRLHNDSSLPTLIIHIRNQDHFRVL QLLCMQTSCYLCSTPMEMGEGAERRKGFISFVSKITLNVPGYDIIKCTGDARADKKSS SDSAALFMLYELEQRGKLIIEETL" BASE COUNT 1419 a 860 c 1044 g 1408 t ORIGIN 1 atgcattctt caaaggagga gatgaactca gcagacccac ctgatatgat gattttagat 61 gacactgatg ctgttatgaa agaaccttct tcatcttcaa aacccaagga cttaaatcct 121 agtaggtatc aattgcaggt gtacgaggtt gcgaagagaa gaaacattat agctgtgttg 181 gatacaggtg taggaaagac aatgattgct gtgatgctca ttaaagattt tggtgaaact 241 atcaagtctg cagacagtaa aaagttgatt gttttcttgg ctcccactgt tcatctagtt 301 aatcaggtat ttgaatatat aaaggaacat acaagtttgg aagttgaaca gtattatgga 361 gcgaaggggg tagatgaatg gaccttggat tgctgggaga aagaagtaaa ggatcatgat 421 gtaagttttc taactagtca gattttatta gatgctttac gaaatgcatt cttgagtcta 481 gagaaggtgt ccttattgat aatagatgag tgtcaccgtg ctactggcaa ccatccatat 541 gcaagactaa tgaaggtcag taactcgcca aagatttttg gaatgacagc atcactgtct 601 cctccaaaat tgattgtgag tatgaacaat gacctcgaat atgttttgct gctgcagata 661 tatactatag aagacagaac agagatggaa gtttatgtgc cttttgctaa agaaagttgt 721 agattttatg atccagccga actttcttgt tcggatttga aagaaatggt agaaatctct 781 tggtcaaaga ccgatatgtc attgtcgaag ttgcaagact cgctgcaact ttcttacaaa 841 gatttagatg ataagcttaa agcactacga aggcgaatat caaatgatca tgctaagatt 901 ttgcattgcc ttgacaatct tggtctcata tgtgcttatg aggttgattg tgctttccta 961 gagaacatca ttgacaccaa agacgaatgt gaagtatata gagaaagtgt ggtgcagtgc 1021 aaaaatttcc tggaggaagt ccgatgctta attggtgctt ctcttcccct tggagataaa 1081 agttttctga attccggatt tgactatgta aaggcagtag atcttggcta tatatctcca 1141 aaaatgcatg aactacttca acttttccaa tcatttgggg aatctaaaca agtatcgtgc 1201 ctgatttttg ttgaaagaat tattaccgct aaagtaattg aaagatttac gaagaaagta 1261 agctgtctat cacatttcgt ggtttcatat atgactggaa gtaatacatc agttgattcc 1321 ctagcaccta aaatgcaaaa ggaaaccttg gagtcatttc gatccgggaa ggtatctcta 1381 ttatttacta ctgatgtggt tgaggaggga attcatgtac caaactgctg ctatgtgata 1441 cgtttcgacc tgccaaagac agtccggagt tatgtacaat ctcgaggaag agctaggcag 1501 aacaattctg aattcatcat gatgcttgag aggggaaatt tgaaacaaag aaatcatata 1561 tacgatttaa tcaggagtga gtattcaatg aaaaacacag caattaaaag ggatcctggt 1621 tctgatctat gcgttcttaa agactgttcc tttgaagaac caaatatttt tgttgtggat 1681 gctacgggag cttcagttac cacagattct gctgttagcc tcatccatag atattgtgga 1741 aagctccctg gtgataagta tgctacgcca aagccaagtt ttgagttcac atcttctgaa 1801 ggattatacg agtgtaaatt aacgttacct gttaatgcag cgtttcaaac aatagttggt 1861 ccaccgtcta ggaattgtca tttggcaaag cagcttgtaa gcttagaagc atgcaagcag 1921 cttcatcaaa tgggtgcctt gaatgatcat cttattccgt ccatcgaaga gccttcagaa 1981 aatgcttgtg tttctaaagt aaaagattcg cattcaggtg caggaactac aaaacggaag 2041 gagcttcacg gaacaacttg cattcaggca ttatgtggaa gctggggaga aaaatccgat 2101 gatgctgatt tctttgccta taaatttgat tttaaatgca gtattgtcac tgtggtttat 2161 tctcaatttg ttcttctaat tgaatcaaag cttgcggatg atgtggggaa tactgaaatg 2221 gatctttact tgattggaaa gacggctaag gctagtgttt ctttctgtgg gcaagtgcgt 2281 ttaaatgcag aacaggtgat gaaagcaaag cgctttcagg aatttttctt taatggcttg 2341 ttcggcaagt tatttgttgg atctaaatca tctggaacac cgagagaatt tctacttcgg 2401 gataaaacaa gttctttgtg gagtccatcc catatgtatt tgcttttacc ccttgaggat 2461 tgttcaacta aggaattgag aataaactgg ccggcaataa cggcttgtac atttgctgca 2521 gactttttaa ataaaaattc tttgttaggc acagatgatg atggaagcaa tccatcatcc 2581 gtgacagact gcaagaaaac caatatgatt cactttgcta acagtttggt tgacattgac 2641 aatcttagaa atatggtagt actggcaatt cacaccggaa gaatctactg catcattgaa 2701 gccatgagtg ataaaactgc caacagttct ttcgatgaag ctgttgataa ggtctcaaca 2761 gggtttgctt ccttctctga atacttcagg aaaaagtatg tgattgtgct gaaacatcct 2821 gaacagcctt tgttgctgtt aaagcagagc cataacccgc acaacttact tgtgaatttt 2881 aatgatgaag gtttttcagc taaagcatca caagctggcg tagttaatga aaaaccacgt 2941 ttccatgtcc acatgccacc tgagcttttg cttgtccttg atgttccagt aagtgttcta 3001 aaatcacttt acttgcttcc atcagtaatg caccggctgg agtcgttaat gttggccaac 3061 caacttagag aagaaatcaa ctttaattct agcaacatcg atataccaag ctcaatgatc 3121 ctggaagcat tgacgacact tggatgttgt gaaagttttt ccatggaaag gctggaattg 3181 cttggggatt cggttctgaa gtacactgtt agctgtacac tttttcttaa atatcccagc 3241 aaacatgagg gacaattatc ttcgaggcgt tcattggcag tttgtaattc aaccctgcat 3301 aagttgggaa ctgttcacat aatacaggga tacattagga atggtgcttt cgatccccgt 3361 cgttgggtag ctccaggaca acgaactctt cgcccggttc cttgcaagtg tggggtggac 3421 tctctagaag ttcctttgga taaaatcttt cagacggaag acccgaaagt taagtttggt 3481 aaaccatgtg atagaggcca cagatggatg tgttcgaaaa ccatatcaga ttgtgttgaa 3541 gctctcatag gagcatacta cgtaagtggt gggcttgttg cttcccttca tgtgatgaag 3601 tggctcgggg ttgatgtcga acttgatccc acgatggtag ctgaagtcat taatcaagcg 3661 tccctacaaa tttatgtccc caataatgaa atccaagaaa tagagtctaa agttggccac 3721 aatttttctg tcaagtttct cttaaaggag gccttgacac atgaatctgc gcttgagttc 3781 tactgttacc aggtagttga atttcttggt gattctgtgt tggacttgct gatcacccaa 3841 tatctctacc acaatcacac tgatatagat ccgggagagt tgactgacct gcggtccgct 3901 tcagttaata atgaaagctt tgcacaagtt gtcgtgcgcc acgacctcta taagcatctt 3961 caacactgct caagtttact atcagaccaa ataagtgaat atgtgcaatc cttaactgaa 4021 tctcagaaca ccagcagatt ggatcccggc ataaaaggtc ccaaggtcat gtgtgacctg 4081 tttgaaagta ttgttggggc aattttgatt gatacgaacc tgaatcttga caaagtctgg 4141 agaattgtcg aaccattgtt atctccaatt gtgacccctg ataagcttga gctgcctccg 4201 cttcgagaac tgaacgaatt atgcgactct cttggatatt ttattaagga aaaatgtaca 4261 aataaaggag aagtggtgca tgctgagctt ctattacagc tcgatcgtga tttgttggtc 4321 ggagaggggt ttgatagaag taaaaaagta gcaaaaggca aggcagcttc aaatctgttg 4381 aagcaacttg aggtacggac tcgattcaac cggcttcaca atgattcttc actacccacc 4441 ttgattattc atataaggaa ccaggatcat ttcagggtgt tacaactact gtgtatgcaa 4501 acctcatgtt atttatgcag cactccgatg gaaatgggtg aaggtgccga gagaagaaaa 4561 gggtttatca gctttgtgtc aaaaatcacc ttgaatgtcc cgggatacga cattatcaaa 4621 tgcaccggag atgcaagagc tgacaaaaaa agttcttcag actctgcagc actgtttatg 4681 ctttacgaac ttgaacaacg tgggaagctc atcatcgagg aaaccttgta a // LOCUS AeDCL4 9329 bp DNA linear PLN 24-DEC-2022 DEFINITION Complete genomic sequence of DCL4 for Cultivated Okra. ACCESSION AeDCL4 VERSION KEYWORDS . SOURCE Abelmoschus esculentus ORGANISM Abelmoschus esculentus Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Malvales; Malvaceae; Malvoideae; Abelmoschus. REFERENCE 1 (bases 1 to 9329) AUTHORS Magdy,M. TITLE Direct Submission JOURNAL Submitted (24-DEC-2022) Genetics, Faculty of Agriculture, Ain Shams University, Hadayek Shubra, No. 68, Cairo, Cairo 11241, Egypt COMMENT Bankit Comment: ALT EMAIL:m.elmosallamy@gmail.com Bankit Comment: TOTAL # OF SEQS:4 ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..9329 /organism="Abelmoschus esculentus" /mol_type="genomic DNA" /cultivar="OEL12" /db_xref="taxon:455045" /country="Egypt" gene 1..9329 /gene="DCL4" CDS join(1..156,368..544,666..776,962..1102,1200..1274, 1720..1785,1986..2114,2166..2294,2514..2645,3005..3100, 3270..3467,3614..3766,3862..4071,4188..4451,4571..4840, 4920..5243,5511..5609,6258..6464,6609..6815,7287..7745, 7874..8146,8289..8915,8992..9108,9180..9329) /gene="DCL4" /codon_start=1 /product="DICER-Like Protein" /translation="MPDCELSTDGVEPSVASTAKAFSPFSLIVEISEEDEAKVEKKEK DPRHIARKYQLELCKKAMEENIIVYLETGCGKTHIAVLLIYELGHLIRKPQKRICIFL APTVALVQQQARVIEDSLDFKVGTFCGNCRHLKNHHDWEKEIKQYEVLVMTPQILLRS LYHCFIRMDLIALLIFDECHHAQSKSNHPYAEIMKVFYDKATVSMLPRIFGMTASPVV GKDASSQANLPKSINSLENLLDAKVYSVGDKDELECFVATPAVTVYDYGPSSCNTIYC CDKLENIKRQCKLLVRKNGDFQSAKNKRKMLNRMHDNIIFCLENLGLWGALQACRLLC NNSERNELIENEGILSDDSVCDQYLAQSADVFALDCKRDGSAHNLSDVEILKEPFFSK KLLRLVGILSSFRLQQNMKCIIFVNRIVTARSLSYILQNLKFLSSWKCHFLVGVHSGL KSMSRKTMKNILEKFRIGEVNLLVATKVGEGLDIQTCCLVIRFDLPETVASFIQSRGR VRMPLSEYAFLVNSANERELNLINNFKKDEDRMNMEITFRTSTEISIGPEERIYTVDS SGASISSGYSISLLHHYCSKLPHDEYFYPKPSFYYFDDTGGTVCNIILPSNAPIPQIA STPQSSVDAAKKDACLNAIEELHKVGALTDHLLPSQNSVLEEEASLVDSDFGSSEDED LCGELHEMLLPAVLKEPWTNLENYVLHSYYIKFIPDPEDRSYKEFALFVKSPLPKEAE RMELDLHLARRRSVMTKLVPSGVAEFKREQIMDALRFQEMFFKVILDRSKLLSEFVPL GNVDFPSSSSSTFYLLLPVILSNCENKVTVDWGIVQRCLSSPLFKTPVEAAKSGSFSS DVCLQLANGCRSIRDVENSLVSDVHLKYPEQPLLRAKPLFKLHNLLHNRKPEESESHE LEEYFIDLPPELCQLKIIGFSKDIESALSLLPSIMHRLENLLVAIELKDVFSASFPEG AEVTARRVLEALTTEKCQERFSLERLESLGDAFLKFAVGRHLFLLHDSLDEGQLTRRR SNAVNNSNLFKLATRRNLQVYIRDQPFDPCQFFALGHPCPVFCTKETKDTVHSQNSCQ VDRANSKVRCSRNQHWLHKKTIADVFEALVVAFIVDRSFQAATAFLRWIGIRVDFQGS QINSICTESKRFIPLSSQVDTEALENLLGYQFLHKGLLLQAIVHPSYNKLGGGCFQRL EFLGDAVLDYLITSYLFSLYPKLKPGQLTDLRSVSVNNKSFANVAVERCLHKFLISDS YPLTEAIEKYVAFFTSSPDRGLFEGPKCPKALGDLVESCFGAILLDTGFDLTRVWKIM LSLLDPIKGLSSVQLSPIREVTELCQSLNWELKFLPSGNGRKFSVDAHAGDVSVSTAE IYTNKKDAIRTAAHQLYVKLKALGYAPKSKSLEEVLKGSIKAEAKLIGFDETPIDVAV PDTTAFRNMTLQESLVNDCNTETRSINKTASTGSPCISPISRAPRSIEAKAGCSASTS CDSQSKGGESHTRTARSQLYEICASNCWNPPLFECCKDEGPSHLRSFTYKVILEIEEA SDMLLECFSSPQTTKKAAAEHAAEGALWYLKHEGYLQ" BASE COUNT 2616 a 1646 c 1771 g 3296 t ORIGIN 1 atgccggatt gcgagttgtc caccgacggg gtcgagccgt ccgttgcctc tacagccaaa 61 gccttttctc ccttttcgct gatcgttgaa atcagcgaag aagatgaagc aaaagtggaa 121 aagaaagaga aggacccacg gcatattgct agaaagttag tcccttttac atttccctat 181 acatgtaaat atttatgttt atttgcttct ttctttcttc tctgtgcaat ggggctctgc 241 tttggttctc ttgatttttt tgttttgttt gttgcatgag ttccagttaa atttaacaag 301 agctctacct tgtttatttc agtttttttg cttagaaatt acattggttt tatgctttta 361 tttaaggtac cagctggagt tgtgcaagaa agccatggaa gagaacataa ttgtatactt 421 ggagactggt tgtgggaaga ctcatattgc tgttctgctt atttatgagc ttggtcattt 481 gatacggaaa cctcagaaaa ggatatgtat ttttcttgct cccacagtgg ctcttgttca 541 gcaggttagc tacttgatct tcaggctaag tttggaatgt aggaactgaa ccggattgtt 601 gattatagat tctatgcagc agtgggacat gcatggtttt gaatgaatct gttactgttt 661 tgtagcaagc ccgtgttata gaagattcac ttgatttcaa ggttgggact ttttgtggta 721 actgcagaca cttgaagaac catcatgatt gggaaaaaga gatcaaacag tatgaggtga 781 ttgtgctctt tgattgatgc aattttaaaa tttatccacg tgttgctcga catcctgtga 841 tggactcttt caattattct ttcttcacct tgtagaaaat ttttacttag gatcatggat 901 cctcgaggat ttgcacgatc atttgcttat gtttctattc atgctgattt ttcttttata 961 ggttcttgtt atgactcctc aaatactact tcgtagttta tatcactgct tcatcaggat 1021 ggacttaata gctcttctga tatttgatga gtgccatcat gctcaaagta aaagcaatca 1081 cccatatgca gaaatcatga aagtaaagtg ctacacatat tgtatttgta ctctcattga 1141 tagatttact ttagtttttt tatcagttga tgtattagat taccttgaca atcatgtagg 1201 ttttttatga taaagctact gtgtcaatgc ttcctcgtat atttggcatg acagcatctc 1261 ccgtagttgg taaaggtaat taaaaagttg catttctttt gaaacaaaag tcgagtcact 1321 ccacgtatca attatgagtt gttcaactcc tttttctgca tactaacata tatttattaa 1381 ctactggctt aaaatgtcat ttaattatgg gtcaacttct tagcttgcaa aatgttaagc 1441 aaaaaacgta acagacatcc ggtgttttca aattgaaaat gttttctctt tttgtttatt 1501 ctcgtatttg aacccatttt catttttgtt ttctaaaaat ttgtttaaat ttagaaataa 1561 cacaatatgt ttcagtttat gcgatacaaa tattttggat tatattgctt cttgtagggc 1621 acttcaatga tatattttct gcctgagtga gagactagtt tttagttatt atctcatatt 1681 ttgatagtta cttctatgat aacctatctt atgctttcag atgcatctag tcaagcaaat 1741 ttaccaaaaa gcatcaacag tctcgaaaac ttgcttgatg ctaaggtgag tcttggaaac 1801 tcactgcgaa atccgatata atcttgtctg ttgatttcct tctaaaaact aattgtgttt 1861 aattttataa attagacttt ttggttataa tcaaaatggg aggggatttt tttgaaggcc 1921 attgctgttc tgatgcagat gctcatagta ggcatttttc taatatttct ccttataagt 1981 tgtaggtcta ttcagttgga gataaggatg aattagaatg ctttgtagca actcctgctg 2041 ttacagtata tgattatggt ccttctagtt gcaacactat ttattgttgt gacaaacttg 2101 agaacattaa gcgtattatc atgaaagaag ctttattttg tttttctgtt tcttcattgc 2161 aagaacagtg caaattactt gtcaggaaga atggtgattt tcaatctgcc aaaaacaaaa 2221 gaaagatgct caatagaatg catgataata taatcttttg cttggaaaat cttggccttt 2281 ggggagcatt acaagtaaga ttttgtgaaa acaactgaat ttcctgtttt tgtaaattgg 2341 cagttgagaa cctttcctct gcaataaaat attctgctgc taaagttatt ttggctctta 2401 tgtcatattc tgcaaccatt ttctgcttat tacccttgca aatttatgtt ggaaatattg 2461 ttggttaaaa tatcttgggt aattctgatt taatctttcc ccgcattttg taggcttgcc 2521 gacttctttg taataactct gagcggaatg aattgataga aaatgaagga attttaagtg 2581 atgactctgt atgtgatcaa tatcttgctc agtctgctga tgtttttgct ttggattgta 2641 agagaggtat aatctgttca ggcattttaa attgtagttt caacttcttt atagctgttg 2701 tcaaatttct tggttgctgc atgcccatct attgtgctca ttgaaattca attatgcagg 2761 agagatatgg taaccggctt agggactttt agtagtagct tcaacttaat tctacttact 2821 gtctctgttt cttgatggtt gcttatgatt tactacttca ttgtatgcat taaacttcaa 2881 ataatgtgta ttattttttt tttgtaaatg gagattctta gataaatttt gaacttttat 2941 gctctgctgc ttgtttcagt taactcgagt aagaatctca gctattcgtc tttgtaatat 3001 tgtagatggg tcagcacata atttatctga tgtagagatt ttgaaggaac catttttctc 3061 gaaaaagctg ttacgccttg ttggaattct ttcctccttc aggtttgtat tcaagcatgt 3121 atattgtttt ttgattgttt gttagctatt cctatgtgga gacatttcaa tggttatagg 3181 attttataaa taaaactctt tattattaac taaaaagtaa ggtgttctat ataaatattt 3241 ctctgacaaa cttctgcaaa tccttttgca ggttacagca aaatatgaaa tgtataattt 3301 ttgtgaatag aattgttact gcaagatcct tgtcgtacat actgcaaaac cttaagttcc 3361 tatcttcttg gaagtgccat tttcttgtgg gagtccactc tggacttaaa agtatgtcaa 3421 gaaagacaat gaaaaacatt ctagagaagt tcagaattgg agaggtaacc cttttctgct 3481 agtgttaata ttccttgctt accctacccc ttccatgcca tagttcacgt aatttaatga 3541 ttccttgttc aatctgtttg actggcagaa cactgattta ttcttgattc cttgaatgcc 3601 gactttgcag gtgaatcttt tggttgcaac taaagttggt gaaggacttg acatccagac 3661 atgctgcctt gtgatacgtt ttgaccttcc agaaactgtt gcaagtttta tccaatcaag 3721 aggtcgtgta agaatgcctc tgtctgagta tgcatttcta gtgaacaggt gctgatcata 3781 aaagtatatg ctatttgaag gtctgtccat atgatttcct ttccgttatt cggaaaaaat 3841 aagttttctt tttcttaatg cagtgctaat gagagggaac taaatttgat taacaatttt 3901 aagaaagatg aagatcgtat gaatatggaa attactttca gaacatctac tgagatttct 3961 attggtcccg aggaaagaat atatacagtt gattcatccg gtgcttcgat tagttcagga 4021 tatagtatct ctctacttca tcattactgc tctaaacttc cccatgatga gtaggccttc 4081 taacttttcc gttcttacct gcttttaaaa ttataatgtc cactacatat gtttgtgtgg 4141 aaattccttt actcatttag attatcctca tgaaaatatt tttcaggtac ttttacccca 4201 agccaagctt ttattatttt gatgatacag gaggaacagt ctgcaatata attttacctt 4261 ccaatgctcc aatacctcaa attgccagta cacctcaatc ttcagttgat gctgccaaaa 4321 aggatgcttg tctaaacgcc attgaagaac tgcataaagt gggagccttg actgaccatc 4381 tcttgccatc gcaaaacagt gttcttgagg aggaagcatc gctggtggat tctgattttg 4441 ggagctctga aggttgatgc ggagcaactt tgttgtcttt ttctttttgt ttggggttgg 4501 ggtgactagt gtagaactaa ttacttttgc ttgtctctaa tctctgctaa atttgatttg 4561 tttgttggta gatgaggatt tgtgtggtga actacatgag atgcttctgc ctgctgtgct 4621 taaagaacca tggactaact tggaaaacta tgtccttcat tcttactata taaaatttat 4681 cccggatcct gaggatagga gctataaaga gttcgcactt tttgtcaagt ctcctctacc 4741 taaagaagct gagagaatgg agctggatct tcacctagct cgccgtaggt cagtgatgac 4801 aaagcttgtc ccatcaggag tagcagaatt taagagagaa gaggtaacta tgttttgttc 4861 tggcagagtt tgttctaatt aacgaactta ccaaaattca ttgtgtattc ctacttatgc 4921 agatcatgga tgcactgcgt tttcaagaaa tgttcttcaa ggtcatcctt gatagatcaa 4981 aactcctttc tgaatttgtt cctttaggaa atgttgactt tccttcatca agctcctcaa 5041 cattctactt gttactccct gttattcttt ccaattgtga gaataaagtg acagtggact 5101 gggggattgt gcaaagatgt ttgtcatctc cactttttaa gactcctgta gaagctgcaa 5161 agagtggaag tttctcttca gatgtttgct tgcaacttgc aaatggttgt aggagtataa 5221 gagacgttga gaatagtttg gtgtatgcca cacacaaggc gaccttttac tttattacta 5281 acattgtcag tgaaaaaaat ggttacagcc cctatagaga ttctggcact ttaaatcata 5341 tggaccactt aaaaaagttg gtagctctca actcttaatt tacaactgtt attagtatta 5401 tccgaaaata gcttgattta taagaggaag gttccacacc aagttgttta gactagcttt 5461 tgatatagta ttcaattgat agagttttaa catcaatgtt gtcaatcagg tctgacgtcc 5521 atctgaagta ccctgaacaa cctcttctgc gtgcaaaacc actttttaaa ctgcacaatt 5581 tgcttcacaa ccgaaaaccg gaggagtcag gtaccttctc ttttgctttg gtttcattct 5641 tgttatggtt ccctttttcc taagtatgtt gcttgttata cttgattatt tgtgttcgaa 5701 atatgatatt ctagaacttg ctaccatctt cagggtatat tgctatttgt tttgtcaggc 5761 tgcattatta tttcattata gggacaagat ttatggttca ttattgattg attagtgctg 5821 cattgccttt agctggcatg caatgcctga tttatagtta attatttgtt attggaaacg 5881 aatcttcccc taatggtaag aaattaccag atttgaagta gcataataat atccatgcac 5941 agcttatttg atgctgaaga gatatcttga acttcattct tttggaaatg agagatgctc 6001 aagttgggtg aatagaatgg agactatgag ttctcacatg gtggtgaaaa ataaagccaa 6061 aaattttctt ttcaggattt ctaggtagta attagaatca ggctattgtg acaatacaac 6121 ccaacaaaaa agagagagga aaagaacaag aatcctttct cattttcttt accagcaaat 6181 caaacgaatg agaaaatgtt ttcttgttga ttttctctgg cttttcattt cgtaaggctg 6241 cttctttgtc gtaaacagaa tcgcatgaac tggaagaata cttcatcgat ttgccccctg 6301 agctttgtca gttgaaaata ataggcttct ccaaagatat tgagagtgca ctttctttgt 6361 taccatcaat aatgcatcgc ctagagaact tgcttgtggc tattgaattg aaggatgttt 6421 tttctgcttc atttcctgag ggagctgaag ttacagccag aagggtaaga cctaactgca 6481 tatggtatta tcaatagtgc tctaggttaa gcatccttta aatttactgt gctaatctaa 6541 tgtttactca ggaaatgcaa acatatgcat gaatgttatc tactactaac agtttgaaat 6601 ggtggtaggt cctagaagca ctcactacag agaagtgcca agagcgtttt tctcttgaaa 6661 ggcttgaatc acttggtgat gccttcctca agtttgctgt tggccgtcat ctttttcttt 6721 tgcatgattc actcgatgaa gggcaattga ctaggagaag gtctaatgct gtaaacaatt 6781 ctaatttgtt caagctagca acaaggcgca atttgcaggt ttgaatgtgt tttagaatgt 6841 gatattcatt aggatttttt tgtggttaag cattatatct tgagaaattt tagcagttgg 6901 tcatcaaact atttaaaaaa catagaattt ttttatgatc ccagcctgta attcttcact 6961 tcttacagat gcggagtagt tgcatcaaat gctaaggtgc acgttcatag ttacttgtct 7021 aacatattaa ttttcttgga cctgtagaca taaaaactgt agaaaaatgt ggtatgtata 7081 aaaatgatta aactaaaaaa caatgcattt ggttagaccc ctcctatccc tttttcccat 7141 tctgggattt ttgggcatat tcaaattctt caagtaaaag tcatttggga tggataattt 7201 gaacccaaga agatttaatg gctactgtat ctttggtatt ttaaagtaaa taattaatat 7261 ttacattgct gtatttccac caatttcagg tttacatacg cgatcaacct ttcgatcctt 7321 gccagttctt tgctctgggc catccctgcc ctgtattttg tacaaaagaa actaaagaca 7381 ctgttcattc ccaaaatagt tgtcaagtag atcgtgcaaa tagcaaagtt agatgcagta 7441 gaaatcaaca ttggctacac aagaaaacaa ttgctgatgt gtttgaggct cttgtagtag 7501 catttatagt tgataggagc ttccaagctg caactgcatt tcttagatgg attggcatac 7561 gagtggactt ccaaggttct caaattaata gtatttgcac tgaaagcaag agattcattc 7621 cactttcctc ccaagtagac actgaagctc ttgaaaattt gttgggttat cagtttcttc 7681 acaagggtct cctccttcaa gcgattgtgc atccttctta taataagctt ggtggaggat 7741 gcttccaggt atgcactatt tctgcttttc tacttgaaga aattaaactt cctttataca 7801 tatttcaccc aacctgtcag ttttataaca ttttactttc tgatctacaa ttagtaatac 7861 tttgatattt tcccagagat tggagtttct tggagatgct gtcttggatt atttaatcac 7921 gtcatattta ttttcactgt atccaaagtt gaagcctggt cagttgactg atttgcgatc 7981 agtgtcagtt aacaacaagt cctttgccaa tgttgcggtg gagagatgcc tacacaaatt 8041 tcttatctct gactcatatc ccctcaccga ggccatagaa aaatatgttg ccttcttcac 8101 atcatcacca gacaggggat tgtttgaagg gccaaaatgt ccgaaggtag caattattat 8161 ctgttttatc ctatgcttca tgcatgcatg ctttcttctt ttttccccct ttataaatgt 8221 gagatataat gaattctagc tccttcaaaa agggttctta acttaatagc tttattgttt 8281 tgatacaggc tcttggtgac ttggtcgagt cctgtttcgg tgctattctc cttgatacag 8341 ggtttgactt gacccgtgtt tggaagataa tgctatccct tttggatcca atcaagggcc 8401 tttcaagtgt gcagcttagt cctatcaggg aagtaacaga actttgccag tctcttaact 8461 gggaattgaa gtttctacca tccggaaatg gcagaaaatt ttcagtggat gcacatgcag 8521 gagatgtttc tgtatccact gctgaaatct acactaacaa aaaagatgct atcagaaccg 8581 ccgcacatca attatatgta aagttaaagg cactaggata tgcacccaag tcgaagtcct 8641 tagaggaagt tttaaaggga agcatcaaag ctgaagccaa gctgattgga tttgatgaaa 8701 caccaattga tgtcgctgtt cctgacacca ctgcatttcg aaacatgaca ttgcaagaat 8761 ctcttgtgaa tgattgcaac acagaaaccc gttccattaa taagacagct tctaccggtt 8821 caccctgcat ctcgcctatt agcagagctc cgcgttctat tgaagcaaaa gcagggtgtt 8881 cagcatcaac tagttgtgac tctcaatcca aaggtcaatg attgttacac tttttttatg 8941 agttgtcagt gtggaacaat tatcttattc atctcttaac ttctcagctc aggtgaatca 9001 catactcgta ctgcgagatc acagttatat gaaatctgtg ccagcaattg ttggaacccc 9061 cctttgttcg aatgttgcaa agacgaagga ccaagccatt tgagatcgta agtatgccgc 9121 tactgctcac ctctttgaat catcgtactc ataccctctg tttaaacttg ttttgcagat 9181 tcacttacaa ggtaattcta gaaatcgaag aagcctcgga tatgctgttg gagtgcttca 9241 gcagtcctca gaccaccaag aaggcagctg ccgagcatgc ggcagaaggt gcattgtggt 9301 acctaaagca tgaaggatac ttacaataa //