Number of Reads Sampled Sobs index on OTU level QZ_T5 QZ_T2 QZ_T4 QZ_CK QZ_T3 400 128.87 150.04 146.536 194.702 176.18 201.68 800 173.728 209.053 189.579 250.349 227.329 235.474 1200 202.769 244.571 227.098 246.51 214.68 238.359 1600 224.682 268.196 255.994 253.45 225.183 246.557 2000 233.78 290.954 275.468 251.916 238.694 259.489 2400 238.565 305.706 287.959 258.659 242.465 266.562 2800 243.917 308.902 294.383 264.424 245.416 276.789 3200 252.014 315.18 298.423 268.704 243.936 286.953 3600 256.884 318.683 302.199 275.014 250.039 296.772 4000 263.016 316.178 299.579 279.335 248.139 305.933 4400 269.767 315.613 304.802 280.05 252.387 307.674 4800 271.499 315.719 306.537 284.705 255.383 314.167 5200 278.025 320.379 308.203 288.665 259.303 320.486 5600 281.743 325.013 312.874 291.339 264.154 325.113 6000 282.186 323.888 320.024 294.853 265.668 326.777 6400 283.293 324.3 322.715 298.279 269.073 325.771 6800 285.219 326.436 322.923 300.569 271.669 330.892 7200 286.466 325.641 323.318 302.994 273.482 333.507 7600 285.696 328.056 326.765 304.041 275.618 337.671 8000 288.052 329.929 327.854 307.714 277.693 339.1 8400 290.949 330.021 329.781 310.189 279.782 340.236 8800 291.556 331.58 330.853 312.343 282.206 340.65 9200 292.046 332.613 331.495 314.401 284.488 343.16 9600 291.167 334.529 332.056 315.879 285.74 343.058 10000 292.048 336.469 333.039 317.085 287.017 343.51 10400 294.266 337.682 333.721 318.744 288.557 345.341 10800 294.632 338.906 335.932 320.203 289.538 346.359 11200 295.695 340.01 336.272 320.945 291.292 347.688 11600 295.592 341.123 335.94 321.859 292.987 348.325 12000 296.648 342.277 337.237 323.364 294.106 349.258 12400 297.845 343.718 337.413 324.172 295.496 349.789 12800 300.078 345.59 337.998 324.993 296.553 350.205 13200 299.175 346.226 339.773 326.452 297.682 350.092 13600 300.407 346.753 338.604 327.379 297.889 350.462 14000 301.023 348.206 339.721 328.495 298.566 350.18 14400 302.137 348.408 340.536 329.321 299.411 350.148 14800 302.591 349.624 340.756 329.954 300.16 350.743 15200 303.225 350.018 341.261 330.632 300.519 351.018 15600 303.862 350.024 341.229 331.466 301.219 351.056 16000 304.364 350.413 341.976 332.308 301.904 351.734 16400 304.718 351.039 342.772 332.373 302.037 352.258 16800 306.009 352.217 343.262 333.012 303.026 352.351 17200 306.145 352.468 344.23 333.608 303.519 352.68 17600 306.616 353.052 345.269 334.278 304.018 353.111 18000 307.382 353.99 346.836 334.663 304.545 353.412 18400 308.306 354.879 347.61 335.212 305.317 353.672 18800 308.763 355.429 348.165 335.171 305.915 354.166 19200 309.572 356.127 348.888 335.803 306.067 354.499 19600 310.048 356.407 349.022 336.567 306.157 355.079 20000 310.291 356.826 349.341 336.895 306.72 355.316 20400 311.403 357.508 350.232 337.021 307.355 355.488 20800 311.659 357.631 350.527 337.438 307.887 355.635 21200 312.135 358.066 350.966 337.74 308.186 355.54 21600 313.092 358.451 351.845 338.205 308.735 355.147 22000 313.247 358.604 352.714 338.537 308.684 355.381 22400 313.686 359.048 353.297 339.226 309.225 355.494 22800 313.556 359.347 353.766 339.586 309.398 355.689 23200 314.024 360.14 353.973 340.101 309.689 355.73 23600 314.264 360.305 354.635 340.454 310.174 355.99 24000 314.973 360.644 355.447 340.429 310.09 355.736 24400 315.654 360.804 355.839 340.743 310.129 355.588 24800 316.165 361.22 356.329 341.072 310.45 355.394 25200 316.73 361.267 357.094 341.778 310.643 355.26 25600 317.424 361.535 356.773 342.07 310.659 355.478 26000 317.778 361.509 357.474 342.25 310.972 355.7 26400 317.571 361.287 357.471 342.195 311.212 356.092 26800 317.977 361.477 358.103 342.626 311.465 356.102 27200 317.939 361.863 358.654 342.8 311.633 356.188 27600 317.879 362.047 359.434 343.162 311.783 356.245 28000 318.225 361.887 360.059 343.458 311.772 356.236 28400 318.676 362.038 360.709 343.673 311.998 356.007 28800 319.075 362.044 361.23 343.746 312.065 356.094 29200 319.641 361.977 361.657 343.726 311.971 356.154 29600 319.887 362.211 362.475 344.048 312.349 356.174 30000 319.793 362.226 363.166 344.242 312.447 356.352 30400 320.434 362.279 363.785 344.309 312.688 356.376 30800 321.033 362.376 363.839 344.6 313.026 356.31 31200 321.578 362.637 364.448 344.662 313.191 356.265 31600 322.271 362.863 364.997 344.504 313.256 356.366 32000 322.823 362.926 365.27 344.499 313.613 356.416 32400 323.204 363.192 365.73 344.795 313.494 356.419 32800 323.189 363.351 365.72 344.778 313.792 356.342 33200 323.265 363.258 365.933 344.849 314.223 356.476 33600 323.509 363.284 366.13 344.926 314.483 356.373 34000 323.88 363.156 366.223 345.136 314.503 356.239 34400 324.079 363.303 366.396 345.439 314.754 356.13 34800 323.955 363.582 366.524 345.493 314.837 356.188 35200 324.285 363.576 367.063 345.566 314.866 356.179 35600 324.621 363.774 367.233 345.518 315.058 356.255 36000 324.523 364.157 366.956 345.434 315.221 356.338 36400 324.834 364.304 367.107 345.505 315.296 356.397 36800 324.743 364.492 367.385 345.641 315.512 356.517 37200 324.877 364.634 367.543 345.671 315.69 356.502 37600 325.37 364.856 367.701 345.739 315.924 356.575 38000 325.265 364.98 367.911 346.095 316.06 356.567 38400 325.627 364.793 368.084 346.412 316.203 356.668 38800 326.112 365.001 368.501 346.293 316.587 356.575 39200 326.466 364.968 368.9 346.471 316.609 356.667 39600 326.77 365.065 369.117 346.494 316.857 356.72 40000 327.072 365.145 369.189 346.602 317.075 356.718 40400 327.308 365.101 369.566 346.579 317.187 356.787 40800 327.49 365.056 369.927 346.604 317.352 356.727 41200 327.61 365.068 369.98 346.681 317.339 356.766 41600 327.651 364.915 370.168 346.629 317.481 356.851 42000 327.681 364.931 370.474 346.555 317.429 356.757 42400 328.06 364.998 370.833 346.699 317.54 356.678 42800 328.349 365.076 371.068 346.627 317.668 356.757 43200 328.361 364.979 371.243 346.704 317.816 356.732 43600 328.493 365.126 371.502 346.791 317.863 356.742 44000 328.706 365.224 371.688 347.07 317.913 356.809 44400 328.997 365.274 371.875 347.139 318.033 356.862 44800 329.103 365.188 372.176 347.116 318.105 357.07 44892 329.212 365.177 372.237 347.049 318.213 357.132