Block Type,96well,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
Chemistry,SYBR_GREEN,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
Experiment File Name,G:\11.骨关节炎课题实验结果\实验的数据\细胞外基质和降解酶成分改变\全部对照组和Aggrecan实验组2022-09-06.eds,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
Experiment Run End Time,Not Started,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
Instrument Type,steponeplus,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
Passive Reference,ROX,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
Well,Sample Name,Target Name,Task,Reporter,Quencher,RQ,RQ Min,RQ Max,Cт,Cт Mean,Cт SD,ΔCт,ΔCт Mean,ΔCт SE,HK Control ΔCт Mean,HK Control ΔCт SE,ΔΔCт,Automatic Ct Threshold,Ct Threshold,Automatic Baseline,Baseline Start,Baseline End,Efficiency,Tm1,Tm2,Tm3,Comments,NOISE,OUTLIERRG,MTP
A1,C1,Aggrecan,UNKNOWN,SYBR,None,,,,27.72872353,27.79663086,0.071332976,,,,,,,TRUE,2.733966554,TRUE,3,19,1,81.50647736,,,,N,N,N
A4,C1,Collagen,UNKNOWN,SYBR,None,1,0.859822392,1.163030863,24.30450821,24.17668915,0.16318354,,-3.619942188,0.089046702,,,0,TRUE,2.837617129,TRUE,3,15,1,87.77831268,65.38015747,,,N,N,Y
A7,C1,Adamts5,UNKNOWN,SYBR,None,1,0.788365006,1.268447995,27.60170555,27.47708321,0.271180958,,-0.319548607,0.140202984,,,0,TRUE,3.284746706,TRUE,3,19,1,81.35792542,,,,N,N,N
A10,C1,Mmp13,UNKNOWN,SYBR,None,1,0.930521965,1.074665666,25.37625694,25.33917618,0.046064056,,-2.457455158,0.042456716,,,0,TRUE,3.284360293,TRUE,3,16,1,79.71395874,,,,N,N,N
B1,C1,Aggrecan,UNKNOWN,SYBR,None,,,,27.77397919,27.79663086,0.071332976,,,,,,,TRUE,2.733966554,TRUE,3,19,1,81.50647736,,,,N,N,N
B4,C1,Collagen,UNKNOWN,SYBR,None,1,0.859822392,1.163030863,23.93940163,24.17668915,0.16318354,,-3.619942188,0.089046702,,,0,TRUE,2.837617129,TRUE,3,15,1,87.6289978,65.23083496,,,N,N,Y
B7,C1,Adamts5,UNKNOWN,SYBR,None,1,0.788365006,1.268447995,27.1691246,27.47708321,0.271180958,,-0.319548607,0.140202984,,,0,TRUE,3.284746706,TRUE,3,18,1,81.20860291,,,,N,N,N
B10,C1,Mmp13,UNKNOWN,SYBR,None,1,0.930521965,1.074665666,25.37735558,25.33917618,0.046064056,,-2.457455158,0.042456716,,,0,TRUE,3.284360293,TRUE,3,16,1,79.71395874,,,,N,N,N
C1,C1,Aggrecan,UNKNOWN,SYBR,None,,,,27.78695488,27.79663086,0.071332976,,,,,,,TRUE,2.733966554,TRUE,3,19,1,81.50647736,66.57128906,,,N,N,Y
C4,C1,Collagen,UNKNOWN,SYBR,None,1,0.859822392,1.163030863,24.20640182,24.17668915,0.16318354,,-3.619942188,0.089046702,,,0,TRUE,2.837617129,TRUE,3,15,1,87.77831268,65.23083496,,,N,N,Y
C7,C1,Adamts5,UNKNOWN,SYBR,None,1,0.788365006,1.268447995,27.78490067,27.47708321,0.271180958,,-0.319548607,0.140202984,,,0,TRUE,3.284746706,TRUE,3,19,1,81.35792542,,,,N,N,N
C10,C1,Mmp13,UNKNOWN,SYBR,None,1,0.930521965,1.074665666,25.28282547,25.33917618,0.046064056,,-2.457455158,0.042456716,,,0,TRUE,3.284360293,TRUE,3,16,1,79.71395874,,,,N,N,N
D1,C1,Aggrecan,UNKNOWN,SYBR,None,,,,27.89686584,27.79663086,0.071332976,,,,,,,TRUE,2.733966554,TRUE,3,19,1,81.35712433,,,,N,N,N
D4,C1,Collagen,UNKNOWN,SYBR,None,1,0.859822392,1.163030863,24.25644302,24.17668915,0.16318354,,-3.619942188,0.089046702,,,0,TRUE,2.837617129,TRUE,3,15,1,87.6289978,,,,N,N,N
D7,C1,Adamts5,UNKNOWN,SYBR,None,1,0.788365006,1.268447995,27.35259819,27.47708321,0.271180958,,-0.319548607,0.140202984,,,0,TRUE,3.284746706,TRUE,3,18,1,81.35792542,,,,N,N,N
D10,C1,Mmp13,UNKNOWN,SYBR,None,1,0.930521965,1.074665666,25.32026482,25.33917618,0.046064056,,-2.457455158,0.042456716,,,0,TRUE,3.284360293,TRUE,3,16,1,79.56460571,,,,N,N,N
E1,C1,Gapdh,UNKNOWN,SYBR,None,1,0.91948843,1.08756125,20.9278183,20.87984276,0.068618417,,-6.916788101,0.049489599,,,0,TRUE,2.658159626,TRUE,3,12,1,85.38962555,,,,N,N,N
E4,S1,Aggrecan,UNKNOWN,SYBR,None,,,,31.97545433,32.36585617,0.3035267,,,,,,,TRUE,2.733966554,TRUE,3,23,1,81.05886841,65.23083496,,,N,N,Y
E5,S2,Aggrecan,UNKNOWN,SYBR,None,,,,32.98012924,33.49930954,0.413414925,,,,,,,TRUE,2.733966554,TRUE,3,24,1,81.05949402,64.93050385,,,N,N,Y
E6,S3,Aggrecan,UNKNOWN,SYBR,None,,,,30.91320992,31.48546982,0.434722215,,,,,,,TRUE,2.733966554,TRUE,3,22,1,80.91015625,65.07984924,,,N,N,Y
F1,C1,Gapdh,UNKNOWN,SYBR,None,1,0.91948843,1.08756125,20.82121849,20.87984276,0.068618417,,-6.916788101,0.049489599,,,0,TRUE,2.658159626,TRUE,3,12,1,85.38962555,,,,N,N,N
F4,S1,Aggrecan,UNKNOWN,SYBR,None,,,,32.68273163,32.36585617,0.3035267,,,,,,,TRUE,2.733966554,TRUE,3,24,1,80.9095459,,,,N,N,N
F5,S2,Aggrecan,UNKNOWN,SYBR,None,,,,33.35560989,33.49930954,0.413414925,,,,,,,TRUE,2.733966554,TRUE,3,24,1,80.91015625,,,,N,N,N
F6,S3,Aggrecan,UNKNOWN,SYBR,None,,,,31.43491745,31.48546982,0.434722215,,,,,,,TRUE,2.733966554,TRUE,3,23,1,81.05949402,,,,N,N,N
G1,C1,Gapdh,UNKNOWN,SYBR,None,1,0.91948843,1.08756125,20.82062912,20.87984276,0.068618417,,-6.916788101,0.049489599,,,0,TRUE,2.658159626,TRUE,3,12,1,85.53897858,65.6751709,,,N,N,Y
G4,S1,Aggrecan,UNKNOWN,SYBR,None,,,,32.30049133,32.36585617,0.3035267,,,,,,,TRUE,2.733966554,TRUE,3,23,1,81.20819092,,,,N,N,N
G5,S2,Aggrecan,UNKNOWN,SYBR,None,,,,33.79307556,33.49930954,0.413414925,,,,,,,TRUE,2.733966554,TRUE,3,25,1,81.20883942,,,,N,N,N
G6,S3,Aggrecan,UNKNOWN,SYBR,None,,,,31.94344521,31.48546982,0.434722215,,,,,,,TRUE,2.733966554,TRUE,3,23,1,81.20883942,,,,N,N,N
H1,C1,Gapdh,UNKNOWN,SYBR,None,1,0.91948843,1.08756125,20.94970512,20.87984276,0.068618417,,-6.916788101,0.049489599,,,0,TRUE,2.658159626,TRUE,3,12,1,85.53897858,,,,N,N,N
H4,S1,Aggrecan,UNKNOWN,SYBR,None,,,,32.50474167,32.36585617,0.3035267,,,,,,,TRUE,2.733966554,TRUE,3,24,1,81.20819092,,,,N,N,N
H5,S2,Aggrecan,UNKNOWN,SYBR,None,,,,33.86841583,33.49930954,0.413414925,,,,,,,TRUE,2.733966554,TRUE,3,25,1,81.20883942,,,,N,N,N
H6,S3,Aggrecan,UNKNOWN,SYBR,None,,,,31.65030861,31.48546982,0.434722215,,,,,,,TRUE,2.733966554,TRUE,3,23,1,81.05949402,,,,N,N,N
,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
Analysis Type,Singleplex,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
Endogenous Control,Aggrecan,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
RQ Min/Max Confidence Level,95.0,,,,,,,,,,,,,,,,,,,,,,,,,,,,,
Reference Sample,C1,,,,,,,,,,,,,,,,,,,,,,,,,,,,,