Setup parameters 

Detection image : Hematoxylin OD 
Requested pixel size : 0.5 µm

Background radius : 8 µm
Median filter radius : 0 µm
Sigma : 1.5 µm
Minimum area : 10 µm²
Maximum area : 400 µm²
Threshold : 0.1
Max background intensity : 2
Split by shape :Checked
Cell parameters :

Cell expansion : 5 µm
Include cell nucleus: Checked
General parameters:
Smooth boundaries : Checked
Make measurements : Checked